Cross-correlated relaxation measurements under adiabatic sweeps: determination of local order in proteins

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14740%2F15%3A00086418" target="_blank" >RIV/00216224:14740/15:00086418 - isvavai.cz</a>
Result on the web
<a href="http://download.springer.com/static/pdf/7/art%253A10.1007%252Fs10858-015-9994-8.pdf?originUrl=http%3A%2F%2Flink.springer.com%2Farticle%2F10.1007%2Fs10858-015-9994-8&token2=exp=1454485862~acl=%2Fstatic%2Fpdf%2F7%2Fart%25253A10.1007%25252Fs10858-015-9994-8" target="_blank" >http://download.springer.com/static/pdf/7/art%253A10.1007%252Fs10858-015-9994-8.pdf?originUrl=http%3A%2F%2Flink.springer.com%2Farticle%2F10.1007%2Fs10858-015-9994-8&token2=exp=1454485862~acl=%2Fstatic%2Fpdf%2F7%2Fart%25253A10.1007%25252Fs10858-015-9994-8</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1007/s10858-015-9994-8" target="_blank" >10.1007/s10858-015-9994-8</a>

Result language
angličtina
Original language name
Cross-correlated relaxation measurements under adiabatic sweeps: determination of local order in proteins
Original language description
Adiabatically swept pulses were originally designed for the purpose of broadband spin inversion. Later, unexpected advantages of their utilization were also found in other applications, such as refocusing to excite spin echoes, studies of chemical exchange or fragment-based drug design. Here, we present new experiments to characterize fast (ps-ns) protein dynamics, which benefit from little-known properties of adiabatic pulses. We developed a strategy for measuring cross-correlated cross-relaxation (CCCR) rates during adiabatic pulses. This experiment provides a linear combination of longitudinal and transverse CCCR rates, which is offset-independent across a typical amide spectrum. The pulse sequence can be recast to provide accurate transverse CCCR rates weighted by the populations of exchanging states. Sensitivity can be improved in systems in slow exchange. Finally, the experiments can be easily modified to yield residue-specific correlation times.
Czech name
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Czech description
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Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
CE - Biochemistry
OECD FORD branch
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Project
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Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year
2015
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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