Molecular architecture of the ribosome-bound Hepatitis C Virus internal ribosomal entry site RNA

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14740%2F15%3A00087262" target="_blank" >RIV/00216224:14740/15:00087262 - isvavai.cz</a>

Result on the web

<a href="http://emboj.embopress.org/content/34/24/3042" target="_blank" >http://emboj.embopress.org/content/34/24/3042</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.15252/embj.201592469" target="_blank" >10.15252/embj.201592469</a>

Result language

angličtina

Original language name

Molecular architecture of the ribosome-bound Hepatitis C Virus internal ribosomal entry site RNA

Original language description

Internal ribosomal entry sites (IRESs) are structured cis-acting RNAs that drive an alternative, cap-independent translation initiation pathway. They are used by many viruses to hijack the translational machinery of the host cell. IRESs facilitate translation initiation by recruiting and actively manipulating the eukaryotic ribosome using only a subset of canonical initiation factor and IRES transacting factors. Here we present cryo-EM reconstructions of the ribosome 80S- and 40S-bound Hepatitis C Virus(HCV) IRES. The presence of four subpopulations for the 80S center dot HCV IRES complex reveals dynamic conformational modes of the complex. At a global resolution of 3.9 angstrom for the most stable complex, a derived atomic model reveals a complex fold of the IRES RNA and molecular details of its interaction with the ribosome. The comparison of obtained structures explains how a modular architecture facilitates mRNA loading and tRNA binding to the P-site.

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

CE - Biochemistry

OECD FORD branch

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Project

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Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2015

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

EMBO Journal

ISSN

0261-4189

e-ISSN

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Volume of the periodical

34

Issue of the periodical within the volume

24

Country of publishing house

US - UNITED STATES

Number of pages

17

Pages from-to

3042-3058

UT code for WoS article

000367918000007

EID of the result in the Scopus database

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