Impact of structural stability of cold adapted Candida antarctica lipase B (CaLB): in relation to pH, chemical and thermal denaturation

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14740%2F15%3A00087271" target="_blank" >RIV/00216224:14740/15:00087271 - isvavai.cz</a>

Result on the web

<a href="http://pubs.rsc.org/en/content/articlepdf/2015/ra/c4ra17093h" target="_blank" >http://pubs.rsc.org/en/content/articlepdf/2015/ra/c4ra17093h</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1039/c4ra17093h" target="_blank" >10.1039/c4ra17093h</a>

Result language

angličtina

Original language name

Impact of structural stability of cold adapted Candida antarctica lipase B (CaLB): in relation to pH, chemical and thermal denaturation

Original language description

The effect of pH on the conformational behavior of Candida antartica lipase B (CaLB) has been monitored by spectroscopic and calorimetric studies. The results obtained from far and near-UV CD, intrinsic fluorescence and ANS binding studies indicate thatCaLB exhibits the characteristic properties of a molten globule in acidic (protonated) conditions at pH 1.4. The molten globule state retained about 67% of its secondary structure with a substantial loss of tertiary structure at pH 1.4. Moreover, equilibrium unfolding studies indicated that the 'molten-globule-like' state unfolds in a non-cooperative manner and is thermodynamically less stable than that of the native state. The molten globule possessed a slightly higher Rh than its native state. The DSCthermogram shows a high heat signal at pH 7.4, and a low heat signal at pH 2.6, and suggests that CaLB is likely to have undergone structural changes during the thermal unfolding. However partially unfolded CaLB at pH 1.

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

CE - Biochemistry

OECD FORD branch

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Project

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Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2015

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

RSC Advances

ISSN

2046-2069

e-ISSN

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Volume of the periodical

5

Issue of the periodical within the volume

26

Country of publishing house

GB - UNITED KINGDOM

Number of pages

17

Pages from-to

20115-20131

UT code for WoS article

000350220400039

EID of the result in the Scopus database

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