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Terminal Restriction Fragments (TRF) Method to Analyze Telomere Lengths

The result's identifiers

  • Result code in IS VaVaI

    <a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14740%2F15%3A00088145" target="_blank" >RIV/00216224:14740/15:00088145 - isvavai.cz</a>

  • Result on the web

    <a href="http://www.bio-protocol.org/pdf/Bio-protocol1671.pdf" target="_blank" >http://www.bio-protocol.org/pdf/Bio-protocol1671.pdf</a>

  • DOI - Digital Object Identifier

    <a href="http://dx.doi.org/10.21769/BioProtoc.1671" target="_blank" >10.21769/BioProtoc.1671</a>

Alternative languages

  • Result language

    angličtina

  • Original language name

    Terminal Restriction Fragments (TRF) Method to Analyze Telomere Lengths

  • Original language description

    Chromosome ends - telomeres - are a focus of intensive research due to their importance for the maintenance of chromosome stability. Their shortening du e to incomplete replication functions as a molecular clock counting the number of cell divi sions, and ultimately results in cell-cycle arrest and cellular senescence. Determination of te lomere lengths is an essential approach in telomere biology for research and diagnostic applications. Term inal Restriction Fragments (TRF) analysis is the oldest approach to analyze telomere lengths and remains the “gold standard” even in current studies. This technique relies on the fact that repeated minisatellite telomeric units do not contain target sites for restri ction enzymes. Consequently, telomeres remain in relatively long fragments (TRF), whereas the genomic DNA is digested into short pieces. Fragments of telomeric DNA are then visualized by hybridization with radioactively labeled telomeric probe.

  • Czech name

  • Czech description

Classification

  • Type

    J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

  • CEP classification

    EB - Genetics and molecular biology

  • OECD FORD branch

Result continuities

  • Project

    Result was created during the realization of more than one project. More information in the Projects tab.

  • Continuities

    P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Others

  • Publication year

    2015

  • Confidentiality

    S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Data specific for result type

  • Name of the periodical

    Bio-protocol

  • ISSN

    2331-8325

  • e-ISSN

  • Volume of the periodical

    5

  • Issue of the periodical within the volume

    23

  • Country of publishing house

    US - UNITED STATES

  • Number of pages

    12

  • Pages from-to

  • UT code for WoS article

  • EID of the result in the Scopus database