Terminal Restriction Fragments (TRF) Method to Analyze Telomere Lengths
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14740%2F15%3A00088145" target="_blank" >RIV/00216224:14740/15:00088145 - isvavai.cz</a>
Result on the web
<a href="http://www.bio-protocol.org/pdf/Bio-protocol1671.pdf" target="_blank" >http://www.bio-protocol.org/pdf/Bio-protocol1671.pdf</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.21769/BioProtoc.1671" target="_blank" >10.21769/BioProtoc.1671</a>
Alternative languages
Result language
angličtina
Original language name
Terminal Restriction Fragments (TRF) Method to Analyze Telomere Lengths
Original language description
Chromosome ends - telomeres - are a focus of intensive research due to their importance for the maintenance of chromosome stability. Their shortening du e to incomplete replication functions as a molecular clock counting the number of cell divi sions, and ultimately results in cell-cycle arrest and cellular senescence. Determination of te lomere lengths is an essential approach in telomere biology for research and diagnostic applications. Term inal Restriction Fragments (TRF) analysis is the oldest approach to analyze telomere lengths and remains the “gold standard” even in current studies. This technique relies on the fact that repeated minisatellite telomeric units do not contain target sites for restri ction enzymes. Consequently, telomeres remain in relatively long fragments (TRF), whereas the genomic DNA is digested into short pieces. Fragments of telomeric DNA are then visualized by hybridization with radioactively labeled telomeric probe.
Czech name
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Czech description
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Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
EB - Genetics and molecular biology
OECD FORD branch
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Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2015
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Bio-protocol
ISSN
2331-8325
e-ISSN
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Volume of the periodical
5
Issue of the periodical within the volume
23
Country of publishing house
US - UNITED STATES
Number of pages
12
Pages from-to
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UT code for WoS article
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EID of the result in the Scopus database
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