Identification of miRNA expression profiles in human mesial temporal lobe epilepsy with hippocampal sclerosis

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14740%2F16%3A00088294" target="_blank" >RIV/00216224:14740/16:00088294 - isvavai.cz</a>
Result on the web
<a href="http://www.epilepsyprague2016.org/" target="_blank" >http://www.epilepsyprague2016.org/</a>
DOI - Digital Object Identifier
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Result language
angličtina
Original language name
Identification of miRNA expression profiles in human mesial temporal lobe epilepsy with hippocampal sclerosis
Original language description
Purpose: The primary aim of this study was to identify specific miRNAs with aberrant expression in hippocampal tissue of mesial temporal lobe epilepsy (mTLE) patients with hippocampal sclerosis (HS). Method: We analyzed miRNA isolated from paraffin embedded tissue slices of hippocampal complex from 22 mTLE +HS patients (14 left-sided, 8 right-sided) and 9 post mortem controls. To characterize the miRNAs highly prone to be involved in the pathological changes in the mTLE+HS brain tissue we performed the expression analysis combining the next generation sequencing (NGS) and novel specific technique for miRNA quantification miQPCR. Results: By both methodical approaches we confirmed significant upregulation of miR-129-1-3p, 129-2-3p, 142-3p, 191-5p, 193b-3p, 195-5p, 374b-5p, 451a, and 490-3p in mTLE+HS patients. The levels of miRNA-184 were significantly higher in control subjects.
Czech name
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Czech description
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Project
<a href="/en/project/GA16-04726S" target="_blank" >GA16-04726S: miRNA and biochemical pathways in epilpesy</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year
2016
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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