Systemic administration of miRNA mimics by liposomal delivery system in animal model of colorectal carcinoma

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14740%2F16%3A00088497" target="_blank" >RIV/00216224:14740/16:00088497 - isvavai.cz</a>

Result on the web

<a href="http://www.biomed.cas.cz/physiolres/pdf/65%20Suppl%204/65_S481.pdf" target="_blank" >http://www.biomed.cas.cz/physiolres/pdf/65%20Suppl%204/65_S481.pdf</a>

DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Systemic administration of miRNA mimics by liposomal delivery system in animal model of colorectal carcinoma

Original language description

MiRNAs are important regulators of gene expression and changes in their levels are linked with various pathological states, including solid tumors. MiR-215 has been identified as a tumor suppressor in colorectal cancer (CRC). Following our previous in vitro and in vivo experiments, the aim of this project was to study the possibility of increasing the levels of miR-215 in tumor cells by systemic administration of miRNA mimics in liposomal delivery system in vivo. By subcutaneous xenotransplantation of human cancer cells to NSG mice, CRC model was established. The treatment [ miR-215 mimics in liposomes (20 and 40 mu g/mouse), control oligonucleotide in liposomes, or saline] was administered repeatedly by i.v. injection via tail-vein. Animals were sacrificed, tumor were dissected and measured by a caliper. Expression of miR-215 in tumors, lungs and liver was quantified by RT-PCR. There was no significant differences in tumor volume and miR-215 expression between all three treatment groups.

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

ED - Physiology

OECD FORD branch

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Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2016

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Physiological Research

ISSN

0862-8408

e-ISSN

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Volume of the periodical

65

Issue of the periodical within the volume

Suppl. 4

Country of publishing house

CZ - CZECH REPUBLIC

Number of pages

8

Pages from-to

"S481"-"S488"

UT code for WoS article

000392029200008

EID of the result in the Scopus database

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