Structure and genome release of Twort-like Myoviridae phage with a double-layered baseplate
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14740%2F16%3A00088711" target="_blank" >RIV/00216224:14740/16:00088711 - isvavai.cz</a>
Result on the web
<a href="http://www.pnas.org/content/113/33/9351" target="_blank" >http://www.pnas.org/content/113/33/9351</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1073/pnas.1605883113" target="_blank" >10.1073/pnas.1605883113</a>
Alternative languages
Result language
angličtina
Original language name
Structure and genome release of Twort-like Myoviridae phage with a double-layered baseplate
Original language description
Bacteriophages from the family Myoviridae use double-layered contractile tails to infect bacteria. Contraction of the tail sheath enables the tail tube to penetrate through the bacterial cell wall and serve as a channel for the transport of the phage genome into the cytoplasm. However, the mechanisms controlling the tail contraction and genome release of phages with "double-layered" baseplates were unknown. We used cryo-electron microscopy to show that the binding of the Twort-like phage phi812 to the Staphylococcus aureus cell wall requires a 210 degrees rotation of the heterohexameric receptor-binding and tripod protein complexes within its baseplate about an axis perpendicular to the sixfold axis of the tail. This rotation reorients the receptor-binding proteins to point away from the phage head, and also results in disruption of the interaction of the tripod proteins with the tail sheath, hence triggering its contraction.
Czech name
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Czech description
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Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
EE - Microbiology, virology
OECD FORD branch
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Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2016
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Proceedings of the National Academy of Sciences of the United States of America
ISSN
0027-8424
e-ISSN
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Volume of the periodical
113
Issue of the periodical within the volume
33
Country of publishing house
US - UNITED STATES
Number of pages
6
Pages from-to
9351-9356
UT code for WoS article
000381399200067
EID of the result in the Scopus database
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