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NMR solution structure determination of large RNA-protein complexes

The result's identifiers

  • Result code in IS VaVaI

    <a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14740%2F16%3A00088765" target="_blank" >RIV/00216224:14740/16:00088765 - isvavai.cz</a>

  • Result on the web

    <a href="http://www.sciencedirect.com/science/article/pii/S0079656516300310" target="_blank" >http://www.sciencedirect.com/science/article/pii/S0079656516300310</a>

  • DOI - Digital Object Identifier

    <a href="http://dx.doi.org/10.1016/j.pnmrs.2016.10.001" target="_blank" >10.1016/j.pnmrs.2016.10.001</a>

Alternative languages

  • Result language

    angličtina

  • Original language name

    NMR solution structure determination of large RNA-protein complexes

  • Original language description

    Structure determination of RNA-protein complexes is essential for our understanding of the multiple layers of RNA-mediated posttranscriptional regulation of gene expression. Over the past 20 years, NMR spectroscopy became a key tool for structural studies of RNA-protein interactions. Here, we review the progress being made in NMR structure determination of large ribonucleoprotein assemblies. We discuss approaches for the design of RNA-protein complexes for NMR structural studies, established and emerging isotope and segmental labeling schemes suitable for large RNPs and how to gain distance restraints from NOEs, PREs and EPR and orientational information from RDCs and SAXS/SANS in such systems. the new combination of NMR measurements with MD simulations and its potential will also be discussed.

  • Czech name

  • Czech description

Classification

  • Type

    J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

  • CEP classification

    CF - Physical chemistry and theoretical chemistry

  • OECD FORD branch

Result continuities

  • Project

    Result was created during the realization of more than one project. More information in the Projects tab.

  • Continuities

    P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Others

  • Publication year

    2016

  • Confidentiality

    S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Data specific for result type

  • Name of the periodical

    PROGRESS IN NUCLEAR MAGNETIC RESONANCE SPECTROSCOPY

  • ISSN

    0079-6565

  • e-ISSN

  • Volume of the periodical

    97

  • Issue of the periodical within the volume

    November

  • Country of publishing house

    GB - UNITED KINGDOM

  • Number of pages

    25

  • Pages from-to

    57-81

  • UT code for WoS article

    000390507100003

  • EID of the result in the Scopus database