High-Throughput Immunogenetics for Clinical and Research Applications in Immunohematology: Potential and Challenges
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14740%2F17%3A00100395" target="_blank" >RIV/00216224:14740/17:00100395 - isvavai.cz</a>
Result on the web
<a href="http://www.jimmunol.org/content/198/10/3765" target="_blank" >http://www.jimmunol.org/content/198/10/3765</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.4049/jimmunol.1602050" target="_blank" >10.4049/jimmunol.1602050</a>
Alternative languages
Result language
angličtina
Original language name
High-Throughput Immunogenetics for Clinical and Research Applications in Immunohematology: Potential and Challenges
Original language description
Analysis and interpretation of Ig and TCR gene rearrangements in the conventional, low-throughput way have their limitations in terms of resolution, coverage, and biases. With the advent of high-throughput, nextgeneration sequencing (NGS) technologies, a deeper analysis of Ig and/or TCR (IG/TR) gene rearrangements is now within reach, which impacts on all main applications of IG/TR immunogenetic analysis. To bridge the generation gap from low-to high-throughput analysis, the EuroClonality-NGS Consortium has been formed, with the main objectives to develop, standardize, and validate the entire workflow of IG/TR NGS assays for 1) clonality assessment, 2) minimal residual disease detection, and 3) repertoire analysis. This concerns the preanalytical (sample preparation, target choice), analytical (amplification, NGS), and postanalytical (immunoinformatics) phases. Here we critically discuss pitfalls and challenges of IG/TR NGS methodology and its applications in hemato-oncology and immunology.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
30102 - Immunology
Result continuities
Project
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Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2017
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Journal of immunology
ISSN
0022-1767
e-ISSN
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Volume of the periodical
198
Issue of the periodical within the volume
10
Country of publishing house
US - UNITED STATES
Number of pages
10
Pages from-to
3765-3774
UT code for WoS article
000401137200001
EID of the result in the Scopus database
2-s2.0-85019694574