Protonation of nucleobases in single- and double-stranded DNA
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14740%2F18%3A00103378" target="_blank" >RIV/00216224:14740/18:00103378 - isvavai.cz</a>
Result on the web
<a href="http://dx.doi.org/10.1002/cbic.201800310" target="_blank" >http://dx.doi.org/10.1002/cbic.201800310</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1002/cbic.201800310" target="_blank" >10.1002/cbic.201800310</a>
Alternative languages
Result language
angličtina
Original language name
Protonation of nucleobases in single- and double-stranded DNA
Original language description
Single-stranded model oligodeoxyribonucleotides containing a single protonable base: cytosine, adenine, guanine, or 5-methylcytosine, centrally located in a background of non-protonable thymines were acid titrated in aqueous solution, with UV-monitoring. The basicity of the central base was shown to depend on the type of the central base and its nearest neighbours and to rise with increasing oligonucleotide length and decreasing ionic strength of the solution. More complex model oligonucleotides containing a centrally located 5-methylcytosine base were comparatively evaluated in single-stranded and double-stranded form, by UV-spectroscopy and high-field NMR. Compared to the single-stranded case, the N3-protonation of the 5-methylcytosine moiety in the double-stranded case occurred at much lower pH, where the duplex already suffered general dissociation. The central guanine:5-methylcytosine base pair remained intact up to this point, possibly due to an unusual alternative protonation on O2 of the 5-methylcytosine moiety already taking place at neutral or weakly basic pH, as indicated by UV spectroscopy, suggesting that in double-stranded DNA, 5-methylcytosine sites may be protonated to a significant extent under physiological conditions.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10400 - Chemical sciences
Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2018
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
ChemBioChem
ISSN
1439-4227
e-ISSN
1439-7633
Volume of the periodical
19
Issue of the periodical within the volume
19
Country of publishing house
DE - GERMANY
Number of pages
11
Pages from-to
2088-2098
UT code for WoS article
000446429400012
EID of the result in the Scopus database
2-s2.0-85052403102