High-Resolution NMR of Folded Proteins in Hyperpolarized Physiological Solvents
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14740%2F18%3A00104510" target="_blank" >RIV/00216224:14740/18:00104510 - isvavai.cz</a>
Result on the web
<a href="http://dx.doi.org/10.1002/chem.201802885" target="_blank" >http://dx.doi.org/10.1002/chem.201802885</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1002/chem.201802885" target="_blank" >10.1002/chem.201802885</a>
Alternative languages
Result language
angličtina
Original language name
High-Resolution NMR of Folded Proteins in Hyperpolarized Physiological Solvents
Original language description
Hyperpolarized 2D exchange spectroscopy (HYPEX) to obtain high-resolution nuclear magnetic resonance (NMR) spectra of folded proteins under near-physiological conditions is reported. The technique is based on hyperpolarized water, which is prepared by dissolution dynamic nuclear polarization and mixed in situ in an NMR spectrometer with a protein in a physiological saline buffer at body temperature. Rapid exchange of labile protons with the hyperpolarized solvent, combined with cross-relaxation effects (NOEs), leads to boosted signal intensities for many amide H-1-N-15 correlations in the protein ubiquitin. As the introduction of hyperpolarization to the target protein is mediated via the solvent, the method is applicable to a broad spectrum of target molecules.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10402 - Inorganic and nuclear chemistry
Result continuities
Project
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Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2018
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Chemistry - A European Journal
ISSN
0947-6539
e-ISSN
1521-3765
Volume of the periodical
24
Issue of the periodical within the volume
51
Country of publishing house
DE - GERMANY
Number of pages
6
Pages from-to
13418-13423
UT code for WoS article
000444413700006
EID of the result in the Scopus database
2-s2.0-85053232886