Characterization of the canine immunoglobulin heavy chain repertoire by next generation sequencing

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14740%2F18%3A00106638" target="_blank" >RIV/00216224:14740/18:00106638 - isvavai.cz</a>

Result on the web

<a href="http://dx.doi.org/10.1016/j.vetimm.2018.07.002" target="_blank" >http://dx.doi.org/10.1016/j.vetimm.2018.07.002</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.vetimm.2018.07.002" target="_blank" >10.1016/j.vetimm.2018.07.002</a>

Result language

angličtina

Original language name

Characterization of the canine immunoglobulin heavy chain repertoire by next generation sequencing

Original language description

The ability to mount adaptive immune responses to a diverse array of pathogens is essential to maintaining the health of an individual. The outcome of adaptive immune responses is influenced by the pool of available lymphocyte antigen receptors. Understanding the composition and dynamics of immune repertoires is hence of relevance to characterizing physiologic immunological processes as well as understanding disease pathogenesis. The dog is increasingly recognized as a model for human disease. The objective of this study was to utilize NGS for comprehensive and unbiased analysis of the IGH repertoire in healthy dogs. First, the IGH locus was searched in silico for previously unidentified genes. Second, IGH transcripts from major lymphoid organs were amplified using a 5'RACE approach without V/J primer bias. Third, amplicons were sequenced on an Illumine MiSeq platform, and data were analyzed using the ARResT/Interrogate platform. Data analysis included V/J usage, V-J pairing biases, isotype frequency, CDR3 diversity, convergent recombination, and public repertoires. The results of this study provide a comprehensive IGH repertoire analysis for healthy dogs. These data will allow further improvement of V/J gene-specific primer sets and will serve as baseline for future studies investigating immune repertoires in health and disease.

Czech name

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Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

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OECD FORD branch

30102 - Immunology

Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2018

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

VETERINARY IMMUNOLOGY AND IMMUNOPATHOLOGY

ISSN

0165-2427

e-ISSN

—

Volume of the periodical

202

Issue of the periodical within the volume

AUG

Country of publishing house

NL - THE KINGDOM OF THE NETHERLANDS

Number of pages

10

Pages from-to

181-190

UT code for WoS article

000442189700024

EID of the result in the Scopus database

2-s2.0-85050262691