A Surprising Photoactivity of Blue Fluorescent Protein TagBFP Allows for Super-Resolution Microscopy
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14740%2F18%3A00106647" target="_blank" >RIV/00216224:14740/18:00106647 - isvavai.cz</a>
Result on the web
<a href="https://journals.aps.org/prl/abstract/10.1103/PhysRevLett.120.233901" target="_blank" >https://journals.aps.org/prl/abstract/10.1103/PhysRevLett.120.233901</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.17691/stm2018.10.1.04" target="_blank" >10.17691/stm2018.10.1.04</a>
Alternative languages
Result language
angličtina
Original language name
A Surprising Photoactivity of Blue Fluorescent Protein TagBFP Allows for Super-Resolution Microscopy
Original language description
The aim of this research was to study photobehavior of a popular blue fluorescent protein TagBFP and apply this marker for super-resolution microscopy. Materials and Methods. Photoactivation of TagBFP was examined both in protein solution in vitro and in living cells. Subdiffraction imaging was performed using total internal reflection fluorescence microscopy followed by super-resolution radial fluctuations or single-molecule localization analysis. Results. We show that TagBFP exhibits blinking behavior upon 405 nm light illumination. Moreover, photoactivation to red-emitting state is occurring in the conditions typically used for TagBFP imaging. The red (photoactivated) form of TagBFP possesses spectral properties similar to TagRFP - a close homologue of TagBFP. We show that both blinking and photoactivation of TagBFP can be utilized for super-resolution imaging. We conclude that photoactivation of TagBFP to red-emitting form should be taken into account in the design of multi-channel imaging experiments involving high-power or prolonged UV illumination.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
30102 - Immunology
Result continuities
Project
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Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2018
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
SOVREMENNYE TEHNOLOGII V MEDICINE
ISSN
2076-4243
e-ISSN
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Volume of the periodical
10
Issue of the periodical within the volume
1
Country of publishing house
RU - RUSSIAN FEDERATION
Number of pages
4
Pages from-to
35-38
UT code for WoS article
000431017800004
EID of the result in the Scopus database
2-s2.0-85044465258