PHOTON-UPCONVERTING NANOPARTICLES AS A NOVEL BACKGROUND-FREE LABEL IN IMMUNOASSAYS

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14740%2F18%3A00113779" target="_blank" >RIV/00216224:14740/18:00113779 - isvavai.cz</a>
Alternative codes found
RIV/68081715:_____/18:00494132
Result on the web
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DOI - Digital Object Identifier
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Result language
angličtina
Original language name
PHOTON-UPCONVERTING NANOPARTICLES AS A NOVEL BACKGROUND-FREE LABEL IN IMMUNOASSAYS
Original language description
Photon-upconverting nanoparticles (UCNPs) have become an attractive label in immunoassays because their anti-Stokes luminescence can be excited by the NIR laser and detected in the VIS region without optical background interference. Further advantages of UCNPs include good photostability, large anti-Stokes shifts, and multiple narrow emission bands that can be used for multiplexed detection. We have developed a competitive upconversion-linked immunosorbent assay (ULISA) for detection of the pharmaceutical diclofenac (DCF) in surface waters. Silica-coated UCNPs (50 nm in diameter) with carboxyl groups on the surface were synthesized and conjugated with the secondary anti-IgG antibody. The structure and monodispersity of the nanoconjugates was studied by TEM and agarose gel electrophoresis. Using a highly affine anti-DCF primary antibody, the optimized ULISA provided a detection limit of 50 pg.mL(-1).
Czech name
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Czech description
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Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year
2018
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Article name in the collection
9TH INTERNATIONAL CONFERENCE ON NANOMATERIALS - RESEARCH & APPLICATION (NANOCON 2017)
ISBN
9788087294819
ISSN
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e-ISSN
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Number of pages
6
Pages from-to
455-460
Publisher name
TANGER LTD
Place of publication
SLEZSKA
Event location
Brno, CZECH REPUBLIC
Event date
Oct 18, 2017
Type of event by nationality
EUR - Evropská akce
UT code for WoS article
000452823300074

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