Phage tail fibre assembly proteins employ a modular structure to drive the correct folding of diverse fibres
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14740%2F19%3A00113245" target="_blank" >RIV/00216224:14740/19:00113245 - isvavai.cz</a>
Result on the web
<a href="http://dx.doi.org/10.1038/s41564-019-0477-7" target="_blank" >http://dx.doi.org/10.1038/s41564-019-0477-7</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1038/s41564-019-0477-7" target="_blank" >10.1038/s41564-019-0477-7</a>
Alternative languages
Result language
angličtina
Original language name
Phage tail fibre assembly proteins employ a modular structure to drive the correct folding of diverse fibres
Original language description
Phage tail fibres are elongated protein assemblies capable of specific recognition of bacterial surfaces during the first step of viral infection(1-4). The folding of these complex trimeric structures often requires a phage-encoded tail fibre assembly (Tfa) proteins(5-7). Despite the wide occurrence of Tfa proteins, their functional mechanism has not been elucidated. Here, we investigate the tail fibre and Tfa of Escherichia coli phage Mu. We demonstrate that Tfa forms a stable complex with the tail fibre, and present a 2.1 angstrom resolution X-ray crystal structure of this complex. We find that Tfa proteins are comprised of two domains: a non-conserved N-terminal domain that binds to the C-terminal region of the fibre and a conserved C-terminal domain that probably mediates fibre oligomerization and assembly. Tfa forms rapidly exchanging multimers on its own, but not a stable trimer, implying that Tfa does not specify the trimeric state of the fibre. We propose that the key conserved role of Tfa is to ensure that fibre assembly and multimerization initiates at the C terminus, ensuring that the intertwined and repetitive structural elements of fibres come together in the correct sequence. The universal importance of correctly aligning the C termini of phage fibres is highlighted by our work.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10606 - Microbiology
Result continuities
Project
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Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2019
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Nature Microbiology
ISSN
2058-5276
e-ISSN
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Volume of the periodical
4
Issue of the periodical within the volume
10
Country of publishing house
GB - UNITED KINGDOM
Number of pages
9
Pages from-to
1645-1653
UT code for WoS article
000487286800010
EID of the result in the Scopus database
2-s2.0-85068091527