Abiotic stress-mediated modulation of the chromatin landscape in Arabidopsis thaliana

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14740%2F20%3A00114766" target="_blank" >RIV/00216224:14740/20:00114766 - isvavai.cz</a>

Result on the web

<a href="https://academic.oup.com/jxb/article/71/17/5280/5856113" target="_blank" >https://academic.oup.com/jxb/article/71/17/5280/5856113</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1093/jxb/eraa286" target="_blank" >10.1093/jxb/eraa286</a>

Result language

angličtina

Original language name

Abiotic stress-mediated modulation of the chromatin landscape in Arabidopsis thaliana

Original language description

Limited information is available on abiotic stress-mediated alterations of chromatin conformation influencing gene expression in plants. In order to characterize the effect of abiotic stresses on changes in chromatin conformation, we employed FAIRE-seq (formaldehyde-assisted isolation of regulatory element sequencing) and DNase-seq to isolate accessible regions of chromatin from Arabidopsis thaliana seedlings exposed to either heat, cold, salt, or drought stress. Approximately 25% of regions in the Arabidopsis genome were captured as open chromatin, the majority of which included promoters and exons. A large proportion of chromatin regions apparently did not change their conformation in response to any of the four stresses. Digital footprints present within these regions had differential enrichment of motifs for binding of 43 different transcription factors. Further, in contrast to drought and salt stress, both high and low temperature treatments resulted in increased accessibility of the chromatin. Also, pseudogenes attained increased chromatin accessibility in response to cold and drought stresses. The highly accessible and inaccessible chromatin regions of seedlings exposed to drought stress correlated with the Ser/Thr protein kinases (MLK1 and MLK2)-mediated reduction and increase in H3 phosphorylation (H3T3Ph), respectively. The presented results provide a deeper understanding of abiotic stress-mediated chromatin modulation in plants.

Czech name

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Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

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OECD FORD branch

10611 - Plant sciences, botany

Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2020

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Journal of Experimental Botany

ISSN

0022-0957

e-ISSN

—

Volume of the periodical

71

Issue of the periodical within the volume

17

Country of publishing house

GB - UNITED KINGDOM

Number of pages

14

Pages from-to

5280-5293

UT code for WoS article

000573252000014

EID of the result in the Scopus database

2-s2.0-85096368940