Cryo-EM of elongating ribosome with EF-Tu center dot GTP elucidates tRNA proofreading

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14740%2F20%3A00118257" target="_blank" >RIV/00216224:14740/20:00118257 - isvavai.cz</a>

Result on the web

<a href="https://www.nature.com/articles/s41586-020-2447-x" target="_blank" >https://www.nature.com/articles/s41586-020-2447-x</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1038/s41586-020-2447-x" target="_blank" >10.1038/s41586-020-2447-x</a>

Result language

angličtina

Original language name

Cryo-EM of elongating ribosome with EF-Tu center dot GTP elucidates tRNA proofreading

Original language description

Ribosomes accurately decode mRNA by proofreading each aminoacyl-tRNA that is delivered by the elongation factor EF-Tu(1). To understand the molecular mechanism of this proofreading step it is necessary to visualize GTP-catalysed elongation, which has remained a challenge(2-4). Here we use time-resolved cryogenic electron microscopy to reveal 33 ribosomal states after the delivery of aminoacyl-tRNA by EF-Tu center dot GTP. Instead of locking cognate tRNA upon initial recognition, the ribosomal decoding centre dynamically monitors codon-anticodon interactions before and after GTP hydrolysis. GTP hydrolysis enables the GTPase domain of EF-Tu to extend away, releasing EF-Tu from tRNA. The 30S subunit then locks cognate tRNA in the decoding centre and rotates, enabling the tRNA to bypass 50S protrusions during accommodation into the peptidyl transferase centre. By contrast, the decoding centre fails to lock near-cognate tRNA, enabling the dissociation of near-cognate tRNA both during initial selection (before GTP hydrolysis) and proofreading (after GTP hydrolysis). These findings reveal structural similarity between ribosomes in initial selection states(5,6)and in proofreading states, which together govern the efficient rejection of incorrect tRNA. Time-resolved cryogenic electron microscopy structures of a ribosome during the delivery of aminoacyl-tRNA by EF-Tu center dot GTP capture 33 ribosomal states, enabling visualization of the initial selection, proofreading and peptidyl transfer stages.

Czech name

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Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

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OECD FORD branch

10608 - Biochemistry and molecular biology

Project

—

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2020

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Nature

ISSN

0028-0836

e-ISSN

—

Volume of the periodical

584

Issue of the periodical within the volume

7822

Country of publishing house

DE - GERMANY

Number of pages

24

Pages from-to

„640“-„+“

UT code for WoS article

000544885800003

EID of the result in the Scopus database

2-s2.0-85087313262