Initial Insights into the Topology of Endoplasmic Reticulum localized PINs in Arabidopsis Thaliana

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14740%2F20%3A00118413" target="_blank" >RIV/00216224:14740/20:00118413 - isvavai.cz</a>
Result on the web
<a href="https://www.ceitec.eu/abstract-book-final-docx-pdf/f36324" target="_blank" >https://www.ceitec.eu/abstract-book-final-docx-pdf/f36324</a>
DOI - Digital Object Identifier
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Result language
angličtina
Original language name
Initial Insights into the Topology of Endoplasmic Reticulum localized PINs in Arabidopsis Thaliana
Original language description
Auxin regulates plant growth and development through differential accumulation within tissues depending on the activity of PIN proteins that facilitate auxin efflux between cells. The plasma membrane PINs are involved in polar auxin transport (PAT), while the ER localized ones regulate intracellular auxin levels. The topology of plasma membrane (PM) localized ("long") PINs has recently been assessed. However, for the ER PINs this has not been done yet and we try to bridge this gap with our recent efforts. For the topology studies we have utilized GFP tagged PIN5 and PIN8 constructs, ectopically localizing on the PM of epidermis and cortex root cells. Here, we present experimental data showing the localization of PIN5 and PIN8 hydrophilic loops (HLs), using approaches based on pH-dependent quenching of fluorescent reporters, immuno-localization and protease protection techniques. The experiments were also guided by topology prediction software.
Czech name
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Czech description
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Project
<a href="/en/project/LQ1601" target="_blank" >LQ1601: CEITEC 2020</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year
2020
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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