Novel Illumina-based next generation sequencing approach with one-round amplification provides early and reliable detection of BCR-ABL1 kinase domain mutations in chronic myeloid leukemia
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14740%2F20%3A00118611" target="_blank" >RIV/00216224:14740/20:00118611 - isvavai.cz</a>
Alternative codes found
RIV/65269705:_____/20:00072731
Result on the web
<a href="https://onlinelibrary.wiley.com/doi/full/10.1111/bjh.16382" target="_blank" >https://onlinelibrary.wiley.com/doi/full/10.1111/bjh.16382</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1111/bjh.16382" target="_blank" >10.1111/bjh.16382</a>
Alternative languages
Result language
angličtina
Original language name
Novel Illumina-based next generation sequencing approach with one-round amplification provides early and reliable detection of BCR-ABL1 kinase domain mutations in chronic myeloid leukemia
Original language description
The occurrence of mutations in the BCR-ABL1 kinase domain (KD) can lead to treatment resistance in chronic myeloid leukaemia patients. Nowadays, next-generation sequencing (NGS) is an alternative method for the detection of kinase domain mutations, compared to routinely used Sanger sequencing, providing a higher sensitivity of mutation detection. However, in the protocols established so far multiple rounds of amplification limit reliable mutation detection to approximately 5% variant allele frequency. Here, we present a simplified, one-round amplification NGS protocol for the Illumina platform, which offers a robust early detection of BCR-ABL1 KD mutations with a reliable detection limit of 3% variant allele frequency.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
30205 - Hematology
Result continuities
Project
<a href="/en/project/NV17-30397A" target="_blank" >NV17-30397A: Mutational screening of primitive cell populations in chronic myeloid leukemia: BCR-ABL1 and beyond</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2020
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
British journal of haematology
ISSN
0007-1048
e-ISSN
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Volume of the periodical
189
Issue of the periodical within the volume
3
Country of publishing house
US - UNITED STATES
Number of pages
6
Pages from-to
469-474
UT code for WoS article
000512165700001
EID of the result in the Scopus database
2-s2.0-85079161451