The coupling mechanism of mammalian respiratory complex I
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14740%2F20%3A00121273" target="_blank" >RIV/00216224:14740/20:00121273 - isvavai.cz</a>
Result on the web
<a href="https://science.sciencemag.org/content/370/6516/eabc4209" target="_blank" >https://science.sciencemag.org/content/370/6516/eabc4209</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1126/science.abc4209" target="_blank" >10.1126/science.abc4209</a>
Alternative languages
Result language
angličtina
Original language name
The coupling mechanism of mammalian respiratory complex I
Original language description
Mitochondrial complex I couples NADH:ubiquinone oxidoreduction to proton pumping by an unknown mechanism. Here, we present cryo-electron microscopy structures of ovine complex I in five different conditions, including turnover, at resolutions up to 2.3 to 2.5 angstroms. Resolved water molecules allowed us to experimentally define the proton translocation pathways. Quinone binds at three positions along the quinone cavity, as does the inhibitor rotenone that also binds within subunit ND4. Dramatic conformational changes around the quinone cavity couple the redox reaction to proton translocation during open-to-closed state transitions of the enzyme. In the induced deactive state, the open conformation is arrested by the ND6 subunit. We propose a detailed molecular coupling mechanism of complex I, which is an unexpected combination of conformational changes and electrostatic interactions.
Czech name
—
Czech description
—
Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
—
OECD FORD branch
10608 - Biochemistry and molecular biology
Result continuities
Project
—
Continuities
—
Others
Publication year
2020
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Science
ISSN
0036-8075
e-ISSN
—
Volume of the periodical
370
Issue of the periodical within the volume
6516
Country of publishing house
US - UNITED STATES
Number of pages
12
Pages from-to
„547“-„+“
UT code for WoS article
000583031800004
EID of the result in the Scopus database
2-s2.0-85094982581