The best of both worlds: Combining lineage-specific and universal bait sets in target-enrichment hybridization reactions

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14740%2F21%3A00119664" target="_blank" >RIV/00216224:14740/21:00119664 - isvavai.cz</a>
Result on the web
<a href="https://bsapubs.onlinelibrary.wiley.com/doi/10.1002/aps3.11438" target="_blank" >https://bsapubs.onlinelibrary.wiley.com/doi/10.1002/aps3.11438</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1002/aps3.11438" target="_blank" >10.1002/aps3.11438</a>

Result language
angličtina
Original language name
The best of both worlds: Combining lineage-specific and universal bait sets in target-enrichment hybridization reactions
Original language description
Premise Researchers adopting target-enrichment approaches often struggle with the decision of whether to use universal or lineage-specific probe sets. To circumvent this quandary, we investigate the efficacy of a simultaneous enrichment by combining universal probes and lineage-specific probes in a single hybridization reaction, to benefit from the qualities of both probe sets with little added cost or effort. Methods and Results Using 26 Brassicaceae libraries and standard enrichment protocols, we compare results from three independent data sets. A large average fraction of reads mapping to the Angiosperms353 (24-31%) and Brassicaceae (35-59%) targets resulted in a sizable reconstruction of loci for each target set (x >= 70%). Conclusions High levels of enrichment and locus reconstruction for the two target sets demonstrate that the sampling of genomic regions can be easily extended through the combination of probe sets in single enrichment reactions. We hope that these findings will facilitate the production of expanded data sets that answer individual research questions and simultaneously allow wider applications by the research community as a whole.
Czech name
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Czech description
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Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10611 - Plant sciences, botany

Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year
2021
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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