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High-Throughput Protein-Nucleic Acid Interaction Assay Based on Protein-Induced Fluorescence Enhancement

The result's identifiers

  • Result code in IS VaVaI

    <a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14740%2F21%3A00119726" target="_blank" >RIV/00216224:14740/21:00119726 - isvavai.cz</a>

  • Result on the web

    <a href="https://link.springer.com/protocol/10.1007/978-1-0716-0935-4_7#citeas" target="_blank" >https://link.springer.com/protocol/10.1007/978-1-0716-0935-4_7#citeas</a>

  • DOI - Digital Object Identifier

    <a href="http://dx.doi.org/10.1007/978-1-0716-0935-4_7" target="_blank" >10.1007/978-1-0716-0935-4_7</a>

Alternative languages

  • Result language

    angličtina

  • Original language name

    High-Throughput Protein-Nucleic Acid Interaction Assay Based on Protein-Induced Fluorescence Enhancement

  • Original language description

    Molecular processes involved in gene expression encompass multitudes of interactions between proteins and nucleic acids. Quantitative description of these interactions is crucial for delineating the mechanisms governing transcription, genome duplication, and translation. Here we describe a detailed protocol for the quantitative analysis of protein-nucleic acid interactions based on protein-induced fluorescence enhancement (PIFE). While PIFE has mainly been used in single-molecule studies, we modified its application for bulk measurement of protein-nucleic acid interactions in microwell plates using standard fluorescent plate readers. The microwell plate PIFE assay (mwPIFE) is simple, does not require laborious protein labeling, and is high throughput. These properties predispose mwPIFE to become a method of choice for routine applications that require multiple parallel measurements such as buffer optimization, competition experiments, or screening chemical libraries for binding modulators.

  • Czech name

  • Czech description

Classification

  • Type

    C - Chapter in a specialist book

  • CEP classification

  • OECD FORD branch

    10608 - Biochemistry and molecular biology

Result continuities

  • Project

    Result was created during the realization of more than one project. More information in the Projects tab.

  • Continuities

    P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Others

  • Publication year

    2021

  • Confidentiality

    S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Data specific for result type

  • Book/collection name

    RNA REMODELING PROTEINS

  • ISBN

    9781071609347

  • Number of pages of the result

    19

  • Pages from-to

    109-117

  • Number of pages of the book

    437

  • Publisher name

    HUMANA PRESS INC

  • Place of publication

    TOTOWA

  • UT code for WoS chapter

    000683504600008