Conformational changes of short tail fibers enable genome delivery of Podoviridae phage SU10
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14740%2F21%3A00123936" target="_blank" >RIV/00216224:14740/21:00123936 - isvavai.cz</a>
Result on the web
<a href="https://pvaconference.wixsite.com/pva2021" target="_blank" >https://pvaconference.wixsite.com/pva2021</a>
DOI - Digital Object Identifier
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Alternative languages
Result language
angličtina
Original language name
Conformational changes of short tail fibers enable genome delivery of Podoviridae phage SU10
Original language description
Escherichia coli phage SU10 belongs to the genus Kuravirusfrom the family Podoviridaeof phages with short tails. Kuraviruseshave the potential to be used in phage therapy against antibiotic-resistant bacterial infections, however, their structure and genome delivery mechanism have not been characterized yet. Here we used cryo-electron microscopy of purified SU10 particles and cryo-electron tomography of infected E. colicells to describe the structural changes of the phage tail that are required for its genome ejection and delivery. The binding of the long tail fibers to the receptors in the outer bacterial membrane is followed by the straightening of nozzle proteins and rotation of short tail fibers by 135°. In the new arrangement, the nozzle proteins and short tail fibers alternate to form a nozzle that prolongs the tail by 28 nm. To open the tail channel, the tail needle detaches from the nozzle proteins. The inner core proteins of five types, one of which has the predicted peptidoglycan-degradation activity, are ejected from the SU10 head before or together with the genome. The nozzle with the putative extension formed by the inner core proteins enables the delivery of the SU10 genome into the bacterial cytoplasm. Tails of podoviridae phages are defined as short and non-contractile, however, here we show that upon infection kuravirus tails extend into a nozzle for genome delivery.
Czech name
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Czech description
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Classification
Type
O - Miscellaneous
CEP classification
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OECD FORD branch
10607 - Virology
Result continuities
Project
<a href="/en/project/LL1906" target="_blank" >LL1906: Phage replication in bacterial biofilm</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2021
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů