14-3-3ζ phosphomimicking mutants: an alternative to phosphorylation?

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14740%2F21%3A00134994" target="_blank" >RIV/00216224:14740/21:00134994 - isvavai.cz</a>
Result on the web
<a href="https://fns.uniba.sk/fileadmin/prif/svk/zborniky/Zbornik2021.pdf" target="_blank" >https://fns.uniba.sk/fileadmin/prif/svk/zborniky/Zbornik2021.pdf</a>
DOI - Digital Object Identifier
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Result language
angličtina
Original language name
14-3-3ζ phosphomimicking mutants: an alternative to phosphorylation?
Original language description
The 14-3-3 proteins are typically found in dimeric form crucial for their proper function in cells. Yet, a unique role of monomers has been debated in recent studies. Monomerization of 14-3-3ζ is regulated via phosphorylation of Ser58 located at dimer interface. However, it is difficult to obtain 14-3-3ζ specifically phosphorylated at Ser58 (pS58) in sufficient purity and amount. For these reasons three distinct phosphomimicking mutants, i. e. 14-3-3ζ S58E, S58D and S57D S58D were designed and studied in terms of the oligomeric nature and thermodynamical properties. Finally, they were compared to 14-3-3ζ wild type and monomeric mutant L12E M78K, used to approximate monomeric 14-3-3ζ pS58, to discuss whether they could be valid alternative for study of 14-3-3ζ pS58. Although phosphomimicking mutants S58E and S58D and 14-3-3ζ pS58 share negative charge at position 58, they were observed to exhibit distinct properties than monomeric 14-3-3ζ. Designed double mutant S57D S58D with additional negative charge in the Ser58 surrounding appeared as more appropriate alternative of pS58 protein.
Czech name
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Czech description
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Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year
2021
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Article name in the collection
Študentská vedecká konferencia PriF UK 2021: zborník recenzovaných príspevkov
ISBN
9788022351324
ISSN
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e-ISSN
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Number of pages
6
Pages from-to
703-708
Publisher name
Univerzita Komenského v Bratislave
Place of publication
Bratislava
Event location
Bratislava
Event date
Apr 21, 2021
Type of event by nationality
EUR - Evropská akce
UT code for WoS article
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