CELLULAR COLOCALIZATION OF TRANSCRIPTION AND TRANSLATION
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14740%2F22%3A00127506" target="_blank" >RIV/00216224:14740/22:00127506 - isvavai.cz</a>
Result on the web
<a href="http://www.ccsss.cz/index.php/ccsss/issue/view/37" target="_blank" >http://www.ccsss.cz/index.php/ccsss/issue/view/37</a>
DOI - Digital Object Identifier
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Alternative languages
Result language
angličtina
Original language name
CELLULAR COLOCALIZATION OF TRANSCRIPTION AND TRANSLATION
Original language description
Free-living bacteria live in competitive and fast-changing natural habitats with non-optimal growing conditions. To survive in response to a variety of stress, they need to have a fast adaptation mechanism for gene expression. The coupling of transcription and translation coordinates optimal gene expression in bacteria. Thus, it could provide a rapid response to the stress induced unstable environment1. The occurrence of coupled transcription-translation (CTT) in bacteria is broadly accepted due to in vitro structures describing CTT complexes2, but in vivo visualization of CTT in living cells is still missing. The bacterial cellular space is very dynamic but highly organized. Recent in vivo single-particle fluorescent studies were focused on the spatial organization of RNA polymerase (RNAP) or ribosomes in the context of nucleoid localization3,4,5. However, these studies monitor separately RNAP or ribosomes and not the whole CTT in bacterial cells. We developed in vivo fluorescent system to monitor CTT in Escherichia coli through a set of strains with fluorescence labelling of proteins (no interference with the CTT function). The system will allow us to monitor the dynamics of this complex system in a single cell in response to different stress conditions and determine the conditions of CTT formation.
Czech name
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Czech description
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Classification
Type
O - Miscellaneous
CEP classification
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OECD FORD branch
10608 - Biochemistry and molecular biology
Result continuities
Project
<a href="/en/project/LX22NPO5103" target="_blank" >LX22NPO5103: National Institute of Virology and Bacteriology</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2022
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů