Viral transcription-translation coupling in mammalian cells
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14740%2F23%3A00132138" target="_blank" >RIV/00216224:14740/23:00132138 - isvavai.cz</a>
Result on the web
<a href="https://cssb.structbio.org/xix-discussions-in-structural-molecular-biology-and-6th-user-meeting-of-ciisb-czech-infrastructure-for-integrative-structural-biology/" target="_blank" >https://cssb.structbio.org/xix-discussions-in-structural-molecular-biology-and-6th-user-meeting-of-ciisb-czech-infrastructure-for-integrative-structural-biology/</a>
DOI - Digital Object Identifier
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Alternative languages
Result language
angličtina
Original language name
Viral transcription-translation coupling in mammalian cells
Original language description
The coupling of transcription and translation (CTT) controls the gene regulation in bacteria. Recent cryo-electron microscopy (cryo-EM) studies showed the physical coupling of these two processes. Interestingly, the double-strand DNA Vaccinia virus (VACV) performs the viral genome replication, transcription, translation, and assembly of virions in infected mammalian cells within discrete cytoplasmic foci called viral factories. The initial rounds of viral transcription and translation during the early phase of infection occur inside the host cytoplasm. In intermediate and late-phase of infection the viral gene expression is carried out inside the viral factories in close association with host ribosomes. Here, we seek to uncover the potential regulation mechanism of viral gene expression via viral-host CTT. The primary approach is to reconstitute the CTT in vitro and use single particle cryo-EM to uncover the detailed view of the structural architecture of the viral-host CTT. We aim to employ cryo-electron tomography and correlated light and electron microscopy (CLEM) to directly visualize the viral factories in a near-native state at a sub-nanometer resolution to confirm the existence of viral-host CTT directly in VACV-infected cells.
Czech name
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Czech description
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Classification
Type
O - Miscellaneous
CEP classification
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OECD FORD branch
10608 - Biochemistry and molecular biology
Result continuities
Project
<a href="/en/project/LL2008" target="_blank" >LL2008: Crosstalk between transcription and translation</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2023
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů