Asymmetric reconstructions of immature tick-borne encephalitis virus particles reveal defects caused by the assembly process

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14740%2F23%3A00132442" target="_blank" >RIV/00216224:14740/23:00132442 - isvavai.cz</a>

Result on the web

<a href="http://www.ccsss.cz/index.php/ccsss/issue/view/41/74" target="_blank" >http://www.ccsss.cz/index.php/ccsss/issue/view/41/74</a>

DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Asymmetric reconstructions of immature tick-borne encephalitis virus particles reveal defects caused by the assembly process

Original language description

Tick-borne encephalitis virus (TBEV) is an enveloped virus belonging to the family Flaviviridae, which causes severe disease of central nervous system in humans. The smooth virion surface is covered by envelope proteins (E-protein), that are together with the membrane proteins (M-protein) anchored in the virus lipid bilayer. During the viral life cycle, the immature non-infectious virus undergoes amaturation process. This process includes proteolytic cleavage of prM and a major reorganization of the envelope proteins on the viral surface.To determine the structure of immature TBEV particles, we purified them from infected tissue culture cells and used cryo-electron microscopy for visualization. The immature particles have “spiky” surface formed by the E-protein-prM-protein complex. We performed single-particle analysis and cryo-electron tomography to reveal the asymmetric nature of the TBEV immature particles. The symmetric, icosahedral, organization of the E-protein-prM-protein spikes on the particle surface is often disrupted by defects introduced during the assembly process of the immature particle. However, these irregularities do not hinder the subsequent maturation process and instead result in mature particles with empty patches in the “herring bone” organization of the mature viral surface.The results provide further insight into the viral maturation process which could be targeted in the future by specific antiviral drugs

Czech name

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Czech description

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Type

O - Miscellaneous

CEP classification

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OECD FORD branch

10607 - Virology

Project

<a href="/en/project/LX22NPO5103" target="_blank" >LX22NPO5103: National Institute of Virology and Bacteriology</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2023

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů