Arabidopsis flippase ALA3 is required for adjustment of early subcellular trafficking in plant response to osmotic stress

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14740%2F23%3A00133365" target="_blank" >RIV/00216224:14740/23:00133365 - isvavai.cz</a>

Result on the web

<a href="https://academic.oup.com/jxb/article/74/17/4959/7206353" target="_blank" >https://academic.oup.com/jxb/article/74/17/4959/7206353</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1093/jxb/erad234" target="_blank" >10.1093/jxb/erad234</a>

Result language

angličtina

Original language name

Arabidopsis flippase ALA3 is required for adjustment of early subcellular trafficking in plant response to osmotic stress

Original language description

To compensate for their sessile lifestyle, plants developed several responses to exogenous changes. One of the previously investigated and not yet fully understood adaptations occurs at the level of early subcellular trafficking, which needs to be rapidly adjusted to maintain cellular homeostasis and membrane integrity under osmotic stress conditions. To form a vesicle, the membrane needs to be deformed, which is ensured by multiple factors, including the activity of specific membrane proteins, such as flippases from the family of P4-ATPases. The membrane pumps actively translocate phospholipids from the exoplasmic/luminal to the cytoplasmic membrane leaflet to generate curvature, which might be coupled with recruitment of proteins involved in vesicle formation at specific sites of the donor membrane. We show that lack of the AMINOPHOSPHOLIPID ATPASE3 (ALA3) flippase activity caused defects at the plasma membrane and trans-Golgi network, resulting in altered endocytosis and secretion, processes relying on vesicle formation and movement. The mentioned cellular defects were translated into decreased intracellular trafficking flexibility failing to adjust the root growth on osmotic stress-eliciting media. In conclusion, we show that ALA3 cooperates with ARF-GEF BIG5/BEN1 and ARF1A1C/BEX1 in a similar regulatory pathway to vesicle formation, and together they are important for plant adaptation to osmotic stress. The interplay of P4-ATPase ALA3, ARF BEX1, and ARF-GEF BEN1 controls vesicle formation at the plasma membrane/trans -Golgi network and the subsequent vesicle trafficking important for the plant response to osmotic stress.

Czech name

—

Czech description

—

Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

—

OECD FORD branch

10611 - Plant sciences, botany

Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2023

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Journal of Experimental Botany

ISSN

0022-0957

e-ISSN

1460-2431

Volume of the periodical

74

Issue of the periodical within the volume

17

Country of publishing house

GB - UNITED KINGDOM

Number of pages

19

Pages from-to

4959-4977

UT code for WoS article

001022967500001

EID of the result in the Scopus database

2-s2.0-85173232821