Structure and replication of <i>Pseudomonas aeruginosa</i> phage JBD30
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14740%2F24%3A00137127" target="_blank" >RIV/00216224:14740/24:00137127 - isvavai.cz</a>
Result on the web
<a href="https://www.embopress.org/doi/epdf/10.1038/s44318-024-00195-1" target="_blank" >https://www.embopress.org/doi/epdf/10.1038/s44318-024-00195-1</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1038/s44318-024-00195-1" target="_blank" >10.1038/s44318-024-00195-1</a>
Alternative languages
Result language
angličtina
Original language name
Structure and replication of <i>Pseudomonas aeruginosa</i> phage JBD30
Original language description
Bacteriophages are the most abundant biological entities on Earth, but our understanding of many aspects of their lifecycles is still incomplete. Here, we have structurally analysed the infection cycle of the siphophage Casadabanvirus JBD30. Using its baseplate, JBD30 attaches to Pseudomonas aeruginosavia the bacterial type IV pilus, whose subsequent retraction brings the phage to the bacterial cell surface. Cryo-electron microscopy structures of the baseplate-pilus complex show that the tripod of baseplate receptor-binding proteins attaches to the outer bacterial membrane. The tripod and baseplate then open to release three copies of the tape-measure protein, an event that is followed by DNA ejection. JBD30 major capsid proteins assemble into procapsids, which expand by 7% in diameter upon filling with phage dsDNA. The DNA-filled heads are finally joined with 180-nm-long tails, which bend easily because flexible loops mediate contacts between the successive discs of major tail proteins. It is likely that the structural features and replication mechanisms described here are conserved among siphophages that utilize the type IV pili for initial cell attachment.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10608 - Biochemistry and molecular biology
Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2024
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
EMBO Journal
ISSN
0261-4189
e-ISSN
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Volume of the periodical
43
Issue of the periodical within the volume
19
Country of publishing house
US - UNITED STATES
Number of pages
22
Pages from-to
4384-4405
UT code for WoS article
001291553200002
EID of the result in the Scopus database
2-s2.0-85204241221