Advanced Imaging and Mechanical Profiling of Biological Samples with Confocal and Atomic Force Microscopy

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14740%2F24%3A00137722" target="_blank" >RIV/00216224:14740/24:00137722 - isvavai.cz</a>
Result on the web
<a href="https://www.nanobiomedconf.com/NBM24/program.php" target="_blank" >https://www.nanobiomedconf.com/NBM24/program.php</a>
DOI - Digital Object Identifier
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Result language
angličtina
Original language name
Advanced Imaging and Mechanical Profiling of Biological Samples with Confocal and Atomic Force Microscopy
Original language description
Atomic Force Microscopy (AFM) traditionally offers high-resolution characterization of surfaces. Scanning the sample with a sharp tip in a piezo-electric controlled manner allows us to reconstruct the morphology of small macromolecules up to whole cells and tissues in a nano-meter scale. The force between the sample and the tip can be finely controlled, and resulting force-distance curves can be used to determine the elastic and viscoelastic properties of the sample. AFM used for studying biological samples are often coupled with optical or confocal microscopes, enhancing the range of imaging modalities. The presented poster will describe basic concepts in AFM technique and mechanical properties, together with real applications of this technique coupled with confocal microscopy. Viewers will see the results of dynamic changes of liposomes stiffness within a cytosol-imitating buffer, a morphological analysis of cells treated with a membrane-intercalating substance, and a mechanical mapping of cells undergoing mitochondrial clustering, confirmed by confocal imaging.
Czech name
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Czech description
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Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year
2024
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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