Evolutionary new centromeres in Arabideae genomes

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14740%2F24%3A00139130" target="_blank" >RIV/00216224:14740/24:00139130 - isvavai.cz</a>

Result on the web

<a href="https://meetings.embo.org/event/24-plant-genome" target="_blank" >https://meetings.embo.org/event/24-plant-genome</a>

DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Evolutionary new centromeres in Arabideae genomes

Original language description

Centromere repositioning is a well-documented phenomenon in mammals and plants. It involves the formation of a new centromere on the same chromosome without disrupting genome colinearity and contributes to genome evolution and reproductive isolation. However, the origin, structure and evolution of evolutionary new centromeres (ENCs) remain largely unexplored. To address this gap, we performed de-novo genome assemblies of six Arabideae species using PacBio HiFi and ONT nanopore reads. For four species (Draba nemorosa, D. podolica, Arabis auriculata, and the early diverging Pseudoturritis turrita) we achieved telomere-to-telomere chromosome-level assemblies, along with scaffold-level assemblies for A. cypria and Pachyneurum grandiflorum. Both Draba species share a common chromosome structure and relative centromere positions, Pa. grandiflorum is structurally similar to Arabis alpina and A. montbretiana, while the genome of A. Auriculate originated by descending dysploidy (from n = 8 to n = 7). In Ps. turrita paleocentromeres consist of four tandem repeats (monomer size 149, 176, 186 and 63 bp) and LTR retroelements (Ale, CRM, Retand). Compared to the paleocentromeres, the ENCs were repositioned by 4 Mb (median) in D. nemorosa, by 17 Mb in D. podolica, by 11 Mb in Pa. grandiflorum and by 6 Mb in A. auriculata. We provide a detailed sequence and epigenetic characterization of homeologous paleocentromeric sites and ENCs and infer the mechanism of centromere repositioning in the Arabideae.

Czech name

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Czech description

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Type

O - Miscellaneous

CEP classification

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OECD FORD branch

10611 - Plant sciences, botany

Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2024

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů