Structural and mechanistic dynamics of Rift Valley Fever Virus replication

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14740%2F25%3A00141468" target="_blank" >RIV/00216224:14740/25:00141468 - isvavai.cz</a>
Result on the web
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DOI - Digital Object Identifier
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Result language
angličtina
Original language name
Structural and mechanistic dynamics of Rift Valley Fever Virus replication
Original language description
Rift Valley fever virus (RVFV) is a zoonotic, segmented RNA virus responsible for severe outbreaks in both humans and livestock. In newborn livestock, RVFV can cause mortality rates of up to 100%, while human fatalities from severe hemorrhagic disease can reach as high as 50%. The virus encodes an RNA-dependent RNA polymerase, known as the L-protein, which is essential for both replication and transcription. The L-protein employs a distinctive prime-and-realign mechanism for internal replication, which is directed by regulatory sequences known as promoters, found at the 5′ and 3′ ends of the single-stranded viral RNAs. However, the mechanistic details of the replication process involving the L-protein of RVFV remain elusive. In this study, we used cryo-electron microscopy (cryo-EM) single-particle analysis to examine the L-protein during replication with various promoter sets. By visualizing the structural intermediates, our findings uncover several conformational changes driven by differential promoter binding. Overall, this study offers valuable insights into the structural and mechanistic dynamics of RVFV replication, enhancing our understanding of how promoter-induced conformational stabilization influences viral RNA synthesis.
Czech name
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Czech description
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Project
<a href="/en/project/LL2008" target="_blank" >LL2008: Crosstalk between transcription and translation</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year
2025
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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