A PROTEO-TRANSCRIPTOMIC APPROACH TO CHARACTERIZE THE HEAT STRESS RESPONSE OF ARABIDOPSIS THALIANA SEEDS

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14740%2F25%3A00143962" target="_blank" >RIV/00216224:14740/25:00143962 - isvavai.cz</a>

Result on the web

<a href="https://csebr.cz/plant-biology-cs-2025/" target="_blank" >https://csebr.cz/plant-biology-cs-2025/</a>

DOI - Digital Object Identifier

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Result language

angličtina

Original language name

A PROTEO-TRANSCRIPTOMIC APPROACH TO CHARACTERIZE THE HEAT STRESS RESPONSE OF ARABIDOPSIS THALIANA SEEDS

Original language description

Here, I present how we used proteomics and transcriptomics methods to decipher the heat stress response of Arabidopsis thaliana seeds at two different developmental stages-two days after pollination and three days after pollination. We examined differential expression to identify genes whose protein and transcript abundances changed, followed by calculating the correlation between changes in protein and transcript levels after heat stress. Additionally, we analyzed genes that were alternatively spliced at the transcriptomic level and searched for peptides that would confirm the translation of these transcript isoforms at the proteomic level. The results show that genes differentially expressed (log2FC &gt; 1 and adj. P. val &lt; 0.05) at both the proteomic and transcriptomic levels are in the minority. Most differentially expressed genes were found to be regulated at either the proteomic or the transcriptomic level, but not both. Despite this, our data show a strong correlation-greater than 0.7-between proteomic and transcriptomic changes. We also demonstrated that a few peptides were detected at the proteomic level, supporting the conclusion that alternative splicing has a measurable impact on protein abundance. The Gene Ontology terms enriched after heat treatment were similar at both transcriptomic and proteomic levels, including terms such as heat stress response and heat stress tolerance. However, the terms enriched among alternatively spliced genes were distinct and did not include heat stress response or tolerance. This suggests that alternative splicing and heat stress are both important for the heat stress 105 response but affect different sets of genes involved in plant growth and development. Furthermore, we observed that even a small developmental difference-just 24 hours between the two stages-resulted in significant variation in differentially expressed and spliced genes. This indicates that the heat stress response differs notably between these two developmental stages in the seed.

Czech name

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Czech description

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Type

O - Miscellaneous

CEP classification

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OECD FORD branch

10611 - Plant sciences, botany

Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2025

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů