Methods for Analyzing Alternative Splicing and Its Regulation in Plants: From Gene-Specific Approaches to Transcriptome-Wide Studies
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14740%2F25%3A00144514" target="_blank" >RIV/00216224:14740/25:00144514 - isvavai.cz</a>
Result on the web
<a href="https://onlinelibrary.wiley.com/doi/10.1111/ppl.70639" target="_blank" >https://onlinelibrary.wiley.com/doi/10.1111/ppl.70639</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1111/ppl.70639" target="_blank" >10.1111/ppl.70639</a>
Alternative languages
Result language
angličtina
Original language name
Methods for Analyzing Alternative Splicing and Its Regulation in Plants: From Gene-Specific Approaches to Transcriptome-Wide Studies
Original language description
Precursor messenger RNA (pre-mRNA) splicing is a fundamental mechanism of gene regulation that influences both mRNA abundance and proteome diversity. In plants, alternative splicing plays a critical role in coordinating development and enabling responses to environmental stress. This process is tightly regulated by the spliceosome and associated splicing factors, which recognize conserved sequence motifs in pre-mRNAs to guide intron removal and exon joining. In this review, we summarize and compare experimental approaches used to analyze both the regulation of alternative splicing and the splicing profiles of genes, spanning from gene-specific assays to transcriptome-wide methods. Gene-specific techniques, such as minigene assays, transient expression systems, electrophoretic mobility shift assays, and isothermal titration calorimetry, provide insights into the molecular interactions between splicing factors and their RNA targets. To identify RNA-binding partners of splicing factors, or splicing factors that interact with a specific RNA, we discuss high-throughput methods that can be applied in vivo and in vitro. By comparing these approaches, we highlight their advantages, limitations, and applications in plant biology. Understanding alternative splicing regulation is essential for deciphering its role in plant adaptation to environmental challenges, with potential implications for crop improvement strategies.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10608 - Biochemistry and molecular biology
Result continuities
Project
<a href="/en/project/GA22-29717S" target="_blank" >GA22-29717S: Unravelling the involvement of PRP8 in mRNA splicing during embryonic development and thermoresponse.</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2025
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
PHYSIOLOGIA PLANTARUM
ISSN
0031-9317
e-ISSN
1399-3054
Volume of the periodical
177
Issue of the periodical within the volume
6
Country of publishing house
US - UNITED STATES
Number of pages
18
Pages from-to
1-18
UT code for WoS article
001617772000001
EID of the result in the Scopus database
2-s2.0-105022220694