Detection of hydrogen peroxide and glucose by enzyme product precipitation on sensor surface
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14750%2F15%3A00081749" target="_blank" >RIV/00216224:14750/15:00081749 - isvavai.cz</a>
Result on the web
<a href="http://dx.doi.org/10.1515/chempap-2015-0003" target="_blank" >http://dx.doi.org/10.1515/chempap-2015-0003</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1515/chempap-2015-0003" target="_blank" >10.1515/chempap-2015-0003</a>
Alternative languages
Result language
angličtina
Original language name
Detection of hydrogen peroxide and glucose by enzyme product precipitation on sensor surface
Original language description
Biocatalysed precipitation of an insoluble product accumulated on the enzyme-modified electrode surface was applied as the amplification path for low concentration sensing of hydrogen peroxide and glucose. Sensitive electrochemical and quartz-crystal microbalance (QCM) biosensors based on biocatalytic precipitation were developed. A horseradish peroxidase (HRP) monolayer-modified electrode was used to sense H2O2 via the oxidation of 4-chloro-1-naphthol (4CN) forming insoluble benzo-4-chlorocyclohexadienone. Additionally, the bienzyme system employed glucose oxidase (GOx) linked to HRP/4CN. The amount of the precipitate assembled on the sensing surface corresponded to the concentration of analytes and to the length of the incubation interval. The precipitated deposits were followed as a change of impedance using cyclic voltammetry (CV), mass change was determined continuously using a microgravimetric quartz-crystal microbalance, and optical microscopy enabled the visualisation of the pr
Czech name
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Czech description
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Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
CE - Biochemistry
OECD FORD branch
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Result continuities
Project
<a href="/en/project/ED1.1.00%2F02.0068" target="_blank" >ED1.1.00/02.0068: Central european institute of technology</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2015
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Chemical papers
ISSN
0366-6352
e-ISSN
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Volume of the periodical
69
Issue of the periodical within the volume
1
Country of publishing house
DE - GERMANY
Number of pages
9
Pages from-to
167-175
UT code for WoS article
000350130100015
EID of the result in the Scopus database
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