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Detection of hydrogen peroxide and glucose by enzyme product precipitation on sensor surface

The result's identifiers

  • Result code in IS VaVaI

    <a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14750%2F15%3A00081749" target="_blank" >RIV/00216224:14750/15:00081749 - isvavai.cz</a>

  • Result on the web

    <a href="http://dx.doi.org/10.1515/chempap-2015-0003" target="_blank" >http://dx.doi.org/10.1515/chempap-2015-0003</a>

  • DOI - Digital Object Identifier

    <a href="http://dx.doi.org/10.1515/chempap-2015-0003" target="_blank" >10.1515/chempap-2015-0003</a>

Alternative languages

  • Result language

    angličtina

  • Original language name

    Detection of hydrogen peroxide and glucose by enzyme product precipitation on sensor surface

  • Original language description

    Biocatalysed precipitation of an insoluble product accumulated on the enzyme-modified electrode surface was applied as the amplification path for low concentration sensing of hydrogen peroxide and glucose. Sensitive electrochemical and quartz-crystal microbalance (QCM) biosensors based on biocatalytic precipitation were developed. A horseradish peroxidase (HRP) monolayer-modified electrode was used to sense H2O2 via the oxidation of 4-chloro-1-naphthol (4CN) forming insoluble benzo-4-chlorocyclohexadienone. Additionally, the bienzyme system employed glucose oxidase (GOx) linked to HRP/4CN. The amount of the precipitate assembled on the sensing surface corresponded to the concentration of analytes and to the length of the incubation interval. The precipitated deposits were followed as a change of impedance using cyclic voltammetry (CV), mass change was determined continuously using a microgravimetric quartz-crystal microbalance, and optical microscopy enabled the visualisation of the pr

  • Czech name

  • Czech description

Classification

  • Type

    J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

  • CEP classification

    CE - Biochemistry

  • OECD FORD branch

Result continuities

  • Project

    <a href="/en/project/ED1.1.00%2F02.0068" target="_blank" >ED1.1.00/02.0068: Central european institute of technology</a><br>

  • Continuities

    P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Others

  • Publication year

    2015

  • Confidentiality

    S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Data specific for result type

  • Name of the periodical

    Chemical papers

  • ISSN

    0366-6352

  • e-ISSN

  • Volume of the periodical

    69

  • Issue of the periodical within the volume

    1

  • Country of publishing house

    DE - GERMANY

  • Number of pages

    9

  • Pages from-to

    167-175

  • UT code for WoS article

    000350130100015

  • EID of the result in the Scopus database