Structural dynamics of Na⁺ and Ca²⁺ interactions with full-size mammalian NCX

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A90242%2F24%3A00139164" target="_blank" >RIV/00216224:90242/24:00139164 - isvavai.cz</a>

Result on the web

<a href="https://www.nature.com/articles/s42003-024-06159-9" target="_blank" >https://www.nature.com/articles/s42003-024-06159-9</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1038/s42003-024-06159-9" target="_blank" >10.1038/s42003-024-06159-9</a>

Result language

angličtina

Original language name

Structural dynamics of Na⁺ and Ca²⁺ interactions with full-size mammalian NCX

Original language description

Cytosolic Ca2+ and Na+ allosterically regulate Na+/Ca2+ exchanger (NCX) proteins to vary the NCX-mediated Ca2+ entry/exit rates in diverse cell types. To resolve the structure-based dynamic mechanisms underlying the ion-dependent allosteric regulation in mammalian NCXs, we analyze the apo, Ca2+, and Na+-bound species of the brain NCX1.4 variant using hydrogen-deuterium exchange mass spectrometry (HDX-MS) and molecular dynamics (MD) simulations. Ca2+ binding to the cytosolic regulatory domains (CBD1 and CBD2) rigidifies the intracellular regulatory loop (5L6) and promotes its interaction with the membrane domains. Either Na+ or Ca2+ stabilizes the intracellular portions of transmembrane helices TM3, TM4, TM9, TM10, and their connecting loops (3L4 and 9L10), thereby exposing previously unappreciated regulatory sites. Ca2+ or Na+ also rigidifies the palmitoylation domain (TMH2), and neighboring TM1/TM6 bundle, thereby uncovering a structural entity for modulating the ion transport rates. The present analysis provides new structure-dynamic clues underlying the regulatory diversity among tissue-specific NCX variants.

Czech name

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Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

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OECD FORD branch

10600 - Biological sciences

Project

—

Continuities

—

Publication year

2024

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Communications Biology

ISSN

2399-3642

e-ISSN

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Volume of the periodical

7

Issue of the periodical within the volume

1

Country of publishing house

DE - GERMANY

Number of pages

14

Pages from-to

1-14

UT code for WoS article

001204554200005

EID of the result in the Scopus database

2-s2.0-85190578632