The in vitro effect of S-adenosyl-L-methionine on the function of rat hepatocytes damaged by D-galactosamine.

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216275%3A25310%2F05%3A00002958" target="_blank" >RIV/00216275:25310/05:00002958 - isvavai.cz</a>

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

The in vitro effect of S-adenosyl-L-methionine on the function of rat hepatocytes damaged by D-galactosamine.

Original language description

Being a key intermediate in transmethylation and transsulphuration reactions, S-adenosyl-L-methionine (SAMe) exerts a possible hepatoprotective effect. We evaluated the ability of SAMe to improve the functions of rat hepatocytes damaged by D-galactosamine (GalN). Hepatocytes were isolated from Wistar rats (220 - 250 g) by collagenase perfusion and cultured in William´s E medium. After cell attachment GalN (40 mM) +/- SAMe (50 and 1000 mg/L) were added to media. Hepatocyte functional capacity and integrity were analysed by albumin secretion activity (ELISA kit, Bethyl Lab.), urea synthesis (kit, Sigma Aldrich) and lactate dehydrogenase (LDH) activity (kit, Sigma Aldrich) in cultivation media after 24 and 48 h incubation. Activities of mitochondrial dehydrogenases were determined by colorimetric assay based on the cleavage of the tetrazolium salt WST-1. GalN increased significantly LDH leakage from the cells during 24 h and 48 h incubation and decreased albumin secretion activity. SAMe s

Czech name

Vliv S-adenosylmethioninu na funkci hepatocytů potkanů poškozených D-galaktosaminem

Czech description

Protože S-adenosylmethionin (SAMe) má možný hepatoprotektivní efekt, zjišťovali jsme schopnost SAMe zlepšit funkci hepatocytů potkanů poškozených D-galaktosaminem (GalN). Izolované hepatocyty potkanů byly umístěny v mediu a byla zjištěna jejich funkční kapacita a integrita (via měření sekrece albuminu, syntesa urey, LDH aktivity a aktivita mitochondriálních dehydrogenas). Z výsledků vyplynulo, že SAMe snižuje toxicitu indukovanou GalN na dávce dependentním způsobem.

Type

D - Article in proceedings

CEP classification

ED - Physiology

OECD FORD branch

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Project

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Continuities

Z - Vyzkumny zamer (s odkazem do CEZ)

Publication year

2005

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Article name in the collection

Clin. Chim. Acta suppl.

ISBN

0009-8981

ISSN

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e-ISSN

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Number of pages

1

Pages from-to

152

Publisher name

Elsevier

Place of publication

UK

Event location

Glasgow, Skotsko

Event date

May 8, 2005

Type of event by nationality

WRD - Celosvětová akce

UT code for WoS article

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