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Voltammetric detection of ovalbumin at screen-printed electrodes in combination with immunomagnetic particles

The result's identifiers

  • Result code in IS VaVaI

    <a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216275%3A25310%2F11%3A39892179" target="_blank" >RIV/00216275:25310/11:39892179 - isvavai.cz</a>

  • Result on the web

  • DOI - Digital Object Identifier

Alternative languages

  • Result language

    angličtina

  • Original language name

    Voltammetric detection of ovalbumin at screen-printed electrodes in combination with immunomagnetic particles

  • Original language description

    Possibility of determination of ovalbumin as a model protein based on ELISA principle is reported in this contribution. Model system consisted of anti-ovalbumin IgG (primary antibody), ovalbumin and anti-ovalbumin-HRP (secondary antibody). After the substrate addition (hydrogen peroxide) current decrease induced by its conversion by HRP has been detected by linear sweep voltammetry. Superparamagnetic microspheres were used for covalent immobilization of primary antibodies which enables better antigen recognization compared to common sorption onto electrode surface.

  • Czech name

  • Czech description

Classification

  • Type

    D - Article in proceedings

  • CEP classification

    CB - Analytical chemistry, separation

  • OECD FORD branch

Result continuities

  • Project

    <a href="/en/project/LC06035" target="_blank" >LC06035: Centre of Biophysical Chemistry, Bioelectrochemistry and Bioanalysis. New Tools for Genomics, Proteomics and Biomedicine.</a><br>

  • Continuities

    P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>Z - Vyzkumny zamer (s odkazem do CEZ)<br>S - Specificky vyzkum na vysokych skolach

Others

  • Publication year

    2011

  • Confidentiality

    S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Data specific for result type

  • Article name in the collection

    Sensing in Electroanalysis Vol. 6

  • ISBN

    978-80-7395-434-5

  • ISSN

  • e-ISSN

  • Number of pages

    10

  • Pages from-to

    347-356

  • Publisher name

    Univerzita Pardubice

  • Place of publication

    Pardubice

  • Event location

    Pardubice

  • Event date

    Nov 14, 2011

  • Type of event by nationality

    EUR - Evropská akce

  • UT code for WoS article