Voltammetric detection of ovalbumin at screen-printed electrodes in combination with immunomagnetic particles
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216275%3A25310%2F11%3A39892179" target="_blank" >RIV/00216275:25310/11:39892179 - isvavai.cz</a>
Result on the web
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DOI - Digital Object Identifier
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Alternative languages
Result language
angličtina
Original language name
Voltammetric detection of ovalbumin at screen-printed electrodes in combination with immunomagnetic particles
Original language description
Possibility of determination of ovalbumin as a model protein based on ELISA principle is reported in this contribution. Model system consisted of anti-ovalbumin IgG (primary antibody), ovalbumin and anti-ovalbumin-HRP (secondary antibody). After the substrate addition (hydrogen peroxide) current decrease induced by its conversion by HRP has been detected by linear sweep voltammetry. Superparamagnetic microspheres were used for covalent immobilization of primary antibodies which enables better antigen recognization compared to common sorption onto electrode surface.
Czech name
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Czech description
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Classification
Type
D - Article in proceedings
CEP classification
CB - Analytical chemistry, separation
OECD FORD branch
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Result continuities
Project
<a href="/en/project/LC06035" target="_blank" >LC06035: Centre of Biophysical Chemistry, Bioelectrochemistry and Bioanalysis. New Tools for Genomics, Proteomics and Biomedicine.</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>Z - Vyzkumny zamer (s odkazem do CEZ)<br>S - Specificky vyzkum na vysokych skolach
Others
Publication year
2011
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Article name in the collection
Sensing in Electroanalysis Vol. 6
ISBN
978-80-7395-434-5
ISSN
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e-ISSN
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Number of pages
10
Pages from-to
347-356
Publisher name
Univerzita Pardubice
Place of publication
Pardubice
Event location
Pardubice
Event date
Nov 14, 2011
Type of event by nationality
EUR - Evropská akce
UT code for WoS article
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