Retention times and bandwidths in reversed-phase gradient liquid
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216275%3A25310%2F11%3A39892628" target="_blank" >RIV/00216275:25310/11:39892628 - isvavai.cz</a>
Result on the web
<a href="http://dx.doi.org/10.1016/j.chroma.2011.06.064" target="_blank" >http://dx.doi.org/10.1016/j.chroma.2011.06.064</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.chroma.2011.06.064" target="_blank" >10.1016/j.chroma.2011.06.064</a>
Alternative languages
Result language
angličtina
Original language name
Retention times and bandwidths in reversed-phase gradient liquid
Original language description
The present work overviews the attempts at implementation of the conventional gradient theory developer for low-molecular compounds to the description and prediction of gradient separations of peptides and proteins on various types of HPLC columns: conventional analytical columns packed with wide-pore fully porous, fused-core superficially porous and non-porous particles; silica-based monolithic columns and organic-polymer poly(alkylmethacrylate) and poly(styrene-divinylbenzene) monolithic columns in capillary and disc formats. The attention is focused on the determination of the parameters necessary to predict gradient retention times (volumes) and bandwidths using the theoretical model equations. The accuracy of the prediction of protein retention ontotally porous columns improves if size exclusion effect is taken into account, but this is not necessary with non-porous or superficially porous particles.
Czech name
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Czech description
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Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
CB - Analytical chemistry, separation
OECD FORD branch
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Result continuities
Project
<a href="/en/project/GA203%2F07%2F0641" target="_blank" >GA203/07/0641: New analytical methods in combined high-capacity phase separation systems</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>Z - Vyzkumny zamer (s odkazem do CEZ)
Others
Publication year
2011
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Journal of Chromatography A
ISSN
0021-9673
e-ISSN
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Volume of the periodical
1218
Issue of the periodical within the volume
49
Country of publishing house
NL - THE KINGDOM OF THE NETHERLANDS
Number of pages
16
Pages from-to
8874-8889
UT code for WoS article
000297776800014
EID of the result in the Scopus database
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