Optimization of Comprehensive Two-Dimensional Gradient Chromatography Coupling In-Line Hydrophilic Interaction and Reversed Phase Liquid Chromatography

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216275%3A25310%2F12%3A39895677" target="_blank" >RIV/00216275:25310/12:39895677 - isvavai.cz</a>
Result on the web
<a href="http://dx.doi.org/10.1016/j.chroma.2012.10.041" target="_blank" >http://dx.doi.org/10.1016/j.chroma.2012.10.041</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.chroma.2012.10.041" target="_blank" >10.1016/j.chroma.2012.10.041</a>

Result language
angličtina
Original language name
Optimization of Comprehensive Two-Dimensional Gradient Chromatography Coupling In-Line Hydrophilic Interaction and Reversed Phase Liquid Chromatography
Original language description
In-line coupled comprehensive HILICxRP systems should offer larger selectivity differences and better two-dimensional orthogonality than coupled RPxRP systems. However, this may not apply for all systems. The HILIC selectivity depends on the mix of selective polar and non-polar interactions with the functional groups, but also with the matrix of polar columns and depends on the sample type. We synthesized a new polar monolithic sulfobetaine polymethacrylate capillary column with excellent efficiency forlow-molecular compounds. When used in the first, HILIC dimension coupled to core-shell or monolithic RP columns in the second dimension, this column provides much improved ortogonality for two-dimensional separations of phenolic and flavonoid compounds,in comparison to silica-bonded Diol, Polyethylene glycol or Zwitterionic columns. We investigated the performance of 11 short 5 cm and 3 cm columns for fast (1-2 min) gradient second-dimension separations. Band broadening or distortion m
Czech name
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Czech description
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Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
CB - Analytical chemistry, separation
OECD FORD branch
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Project
<a href="/en/project/GAP206%2F12%2F0398" target="_blank" >GAP206/12/0398: Two-dimensional separations in liquid media</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>S - Specificky vyzkum na vysokych skolach

Publication year
2012
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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