Columns and Optimum Gradient Conditions for Fast Second-Dimension Separations in Comprehensive Two-Dimensional Liquid Chromatography

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216275%3A25310%2F12%3A39895679" target="_blank" >RIV/00216275:25310/12:39895679 - isvavai.cz</a>

Result on the web

<a href="http://dx.doi.org/10.1002/jssc.201200061" target="_blank" >http://dx.doi.org/10.1002/jssc.201200061</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1002/jssc.201200061" target="_blank" >10.1002/jssc.201200061</a>

Result language

angličtina

Original language name

Columns and Optimum Gradient Conditions for Fast Second-Dimension Separations in Comprehensive Two-Dimensional Liquid Chromatography

Original language description

Gradient elution provides significantly higher peak capacity in comparison to the isocratic elution mode, hence it is very useful in online comprehensive two-dimensional liquid chromatography (LC). We compared suitability of five commercial core-shell columns and one monolithic column for fast gradients in the second LC dimension, where the time of separation is strictly limited by the fraction cycle time. In two-dimensional reversed-phase systems with partially correlated retention, the resolution, thepeak capacity, and the regularity of coverage of the second-dimension retention space can be improved by appropriate adjusting the gradient time and the gradient range to suit the sample properties. We developed a new strategy for adjusting the gradientmobile phase composition range in the second-dimension, employing the retention data of representative sample standards characterizing the sample properties, which can be calibrated using the reference alkylbenzene series. Optimized seco

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

CB - Analytical chemistry, separation

OECD FORD branch

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Project

<a href="/en/project/GAP206%2F12%2F0398" target="_blank" >GAP206/12/0398: Two-dimensional separations in liquid media</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2012

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Journal of Separation Science

ISSN

1615-9306

e-ISSN

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Volume of the periodical

35

Issue of the periodical within the volume

14

Country of publishing house

DE - GERMANY

Number of pages

11

Pages from-to

1712-1722

UT code for WoS article

000306493700003

EID of the result in the Scopus database

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