Retention Mechanism Studies of Selected Amino Acids and Vitamin B6 on HILIC Columns with Evaporative Light Scattering Detection

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216275%3A25310%2F13%3A39896423" target="_blank" >RIV/00216275:25310/13:39896423 - isvavai.cz</a>

Result on the web

<a href="http://link.springer.com/article/10.1007%2Fs10337-013-2502-y" target="_blank" >http://link.springer.com/article/10.1007%2Fs10337-013-2502-y</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1007/s10337-013-2502-y" target="_blank" >10.1007/s10337-013-2502-y</a>

Result language

angličtina

Original language name

Retention Mechanism Studies of Selected Amino Acids and Vitamin B6 on HILIC Columns with Evaporative Light Scattering Detection

Original language description

The goal of the study was to investigate separation mechanism of selected "essential" amino acids (leucine, isoleucine, threonine, tryptophan, proline, and glycine) and vitamin B6 in hydrophilic interaction liquid chromatography (HILIC) with the evaporative light scattering detection. Chromatographic measurements were made on three different HILIC columns: amide-silica (TSK-gel Amide-80), amino-silica (TSK-gel NH2-100), and cross-linked diol (Luna HILIC). The retention behaviour of the analytes was investigated as a function of different binary hydro-organic mobile phases containing 10-90 % (v/v) acetonitrile. The compounds studied were separated under isocratic and gradient conditions. The best results of tested biologically active compounds separation were obtained on the TSK-gel NH2-100 column. TSK-gel NH2 column showed mixed HILIC-ion-exchange mechanism, the highest separation efficiency and better selectivity and resolution for tested analytes than the other studied column, especi

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

CB - Analytical chemistry, separation

OECD FORD branch

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Project

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Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2013

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Chromatographia

ISSN

0009-5893

e-ISSN

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Volume of the periodical

76

Issue of the periodical within the volume

15-16

Country of publishing house

DE - GERMANY

Number of pages

9

Pages from-to

929-937

UT code for WoS article

000322388200006

EID of the result in the Scopus database

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