Multiplex polymerase chain reaction using ethidium monoazide and propidium monoazide for distinguishing viable and dead cells of arcobacters in biofilm
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216275%3A25310%2F13%3A39896521" target="_blank" >RIV/00216275:25310/13:39896521 - isvavai.cz</a>
Result on the web
<a href="http://dx.doi.org/10.1139/cjm-2013-0635" target="_blank" >http://dx.doi.org/10.1139/cjm-2013-0635</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1139/cjm-2013-0635" target="_blank" >10.1139/cjm-2013-0635</a>
Alternative languages
Result language
angličtina
Original language name
Multiplex polymerase chain reaction using ethidium monoazide and propidium monoazide for distinguishing viable and dead cells of arcobacters in biofilm
Original language description
This paper concerns the formation of biofilm in bacteria of the genus Arcobacter. A multiplex polymerase chain reaction (PCR) method was introduced and optimized for detecting biofilm while using the intercalating dyes ethidium monoazide (EMA) and propidium monoazide (PMA), first for analysis of strains of the genus Arcobacter from a collection, and then applied to samples of prepared biofilms. The results of the study indicate considerable variability among species of bacteria within the genus Arcobacter. The EMA?PMA PCR method can distinguish viable cells from dead cells and is therefore suitable for determining the viability of cells.
Czech name
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Czech description
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Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
EE - Microbiology, virology
OECD FORD branch
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Result continuities
Project
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Continuities
S - Specificky vyzkum na vysokych skolach<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2013
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Canadian Journal of Microbiology
ISSN
0008-4166
e-ISSN
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Volume of the periodical
59
Issue of the periodical within the volume
12
Country of publishing house
CA - CANADA
Number of pages
6
Pages from-to
797-802
UT code for WoS article
000328471300004
EID of the result in the Scopus database
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