Comparison of various techniques for the extraction and determination of antioxidants in plants

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216275%3A25310%2F14%3A39897892" target="_blank" >RIV/00216275:25310/14:39897892 - isvavai.cz</a>

Result on the web

<a href="http://dx.doi.org/10.1002/jssc.201301139" target="_blank" >http://dx.doi.org/10.1002/jssc.201301139</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1002/jssc.201301139" target="_blank" >10.1002/jssc.201301139</a>

Result language

angličtina

Original language name

Comparison of various techniques for the extraction and determination of antioxidants in plants

Original language description

The following extraction techniques have been used for extracting antioxidants (apigenin, coumarin, esculetin, umbelliferone, bergapten, quercetin, rutin, scopoletin and xanthotoxin) from plant material: supercritical fluid extraction, pressurized liquidextraction, extraction by means of Soxhlet apparatus, ultrasonic extraction in ultrasonic bath, and by means of ultrasonic probe. The analytical method based on HPLC-UV detection for the determination of selected antioxidants was developed. For all extracts the antioxidant capacity based on the reduction of free 2,2-diphenyl-1-picrylhydrazyl radical was also determined. Comparing all results the ultrasonic probe method using 0.75 g of sample extracted by 50 mL of acetonitrile in water (30%, v/v) for 25min at room temperature and with amplitude at 60% (equal to 90 W) without pulsation was evaluated as the best tool. The most significant indicator demonstrating this statement is the antioxidant capacity expressed as gallic acid equivale

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

CB - Analytical chemistry, separation

OECD FORD branch

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Project

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Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2014

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Journal of Separation Science

ISSN

1615-9306

e-ISSN

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Volume of the periodical

37

Issue of the periodical within the volume

7

Country of publishing house

DE - GERMANY

Number of pages

10

Pages from-to

835-844

UT code for WoS article

000333538600013

EID of the result in the Scopus database

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