Comparison of four cholesterol-based stationary phases for the separation of steroid hormones
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216275%3A25310%2F14%3A39897964" target="_blank" >RIV/00216275:25310/14:39897964 - isvavai.cz</a>
Result on the web
<a href="http://dx.doi.org/10.1002/jssc.201301088" target="_blank" >http://dx.doi.org/10.1002/jssc.201301088</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1002/jssc.201301088" target="_blank" >10.1002/jssc.201301088</a>
Alternative languages
Result language
angličtina
Original language name
Comparison of four cholesterol-based stationary phases for the separation of steroid hormones
Original language description
The chromatographic behavior of steroid hormones on four cholesterol-bonded stationary phases with different structures in binary methanol/water mobile phases was studied. Of the stationary phases tested, the commercially available stationary phases Cogent UDC cholesterol and COSMOSIL cholester provided better separations of steroid hormones in comparison to homemade aminocholesterol and diaminocholesterol stationary phases. The results show that the temperature has a significant influence on the retention and selectivity for steroid hormones separation. The temperature increase may cause changes in the elution order. From the dependences of the retention (ln k) on temperature (1/T), the standard partial molar enthalpy and standard partial molar entropy were calculated and their enthalpic and entropic contributions to the retention were compared. The enthalpic effects principally control the retention mechanism.
Czech name
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Czech description
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Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
CB - Analytical chemistry, separation
OECD FORD branch
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Result continuities
Project
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Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2014
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Journal of Separation Science
ISSN
1615-9306
e-ISSN
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Volume of the periodical
37
Issue of the periodical within the volume
4
Country of publishing house
DE - GERMANY
Number of pages
7
Pages from-to
345-351
UT code for WoS article
000330756500003
EID of the result in the Scopus database
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