Determination of Total Phenolic Content in Selected Wines Using Amperometric Tyrosinase Biosensor Based on Carbon Nanotubes

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216275%3A25310%2F14%3A39898307" target="_blank" >RIV/00216275:25310/14:39898307 - isvavai.cz</a>
Result on the web
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DOI - Digital Object Identifier
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Result language
angličtina
Original language name
Determination of Total Phenolic Content in Selected Wines Using Amperometric Tyrosinase Biosensor Based on Carbon Nanotubes
Original language description
Polyphenols exhibit significant antioxidant properties of wines. Their antioxidant capacity is commonly expressed as the equivalent weight of Trolox which is synthetic water-soluble analog of alfa-tocopherol (vitamin E), and is called "Trolox equivalentantioxidant capacity" (TEAC). This contribution offers a direct determination of TEAC in real samples without the use of free radicals as it is in conventional spectrometric methods. An amperometric tyrosinase biosensor based on carbon paste electrode modified with carbon nanotubes (CPE/CNTs) is presented. The biosensor, CPE/CNTs/Tyrosinase/Nafion, was prepared by immobilization of tyrosinase enzyme together with Nafion on the surface of CPE/CNTs. All key experimental and instrumentals parameters have been investigated and optimal conditions were founded as follows: (i) amount of tyrosinase in Nafion membrane, 3.0 ?g; (ii) operating potential for amperometric detection, -0.25 V; (iii) speed of stirring, aprox. 400 rpm; (iv) supporting e
Czech name
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Czech description
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Publication year
2014
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Article name in the collection
Sborník přednášek mezinárodní odborné konference XXXIV. Moderní elektrochemické metody
ISBN
978-80-905221-2-1
ISSN
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e-ISSN
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Number of pages
5
Pages from-to
176-180
Publisher name
BEST Servis
Place of publication
Ústí nad Labem
Event location
Jetřichovice
Event date
May 19, 2014
Type of event by nationality
EUR - Evropská akce
UT code for WoS article
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