Macroporous cryogel based spin column with immobilized concanavalin A for isolation of glycoproteins
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216275%3A25310%2F15%3A39898280" target="_blank" >RIV/00216275:25310/15:39898280 - isvavai.cz</a>
Alternative codes found
RIV/68081715:_____/15:00434034
Result on the web
<a href="http://dx.doi.org/10.1002/elps.201400413" target="_blank" >http://dx.doi.org/10.1002/elps.201400413</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1002/elps.201400413" target="_blank" >10.1002/elps.201400413</a>
Alternative languages
Result language
angličtina
Original language name
Macroporous cryogel based spin column with immobilized concanavalin A for isolation of glycoproteins
Original language description
In this work we have developed a hydrophilic poly(hydroxyethyl methacrylate-co-poly(ethylene glycol) diacrylate) cryogel placed in the centrifugal filter device. The composition of the polymerization mixture as well as the polymerization conditions wereoptimized in order to prepare a material with bimodal pore size distribution with 20-50 ?m flow through macropores and submicrometer pores in the polymer walls. The optimized, mechanically stable, highly porous, material was used for spin column lectin chromatography. The surface of the monolithic scaffold was activated by epichlorohydrin and used for immobilization of concanavalin A to provide the affinity supports for selective isolation of glycoproteins containing high mannose glycan structures. Theperformance of the developed lectin modified cryogels was evaluated by analyses of glycoprotein mixtures. The efficiency and selectivity of the affinity supports were confirmed by MALDI-MS analysis.
Czech name
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Czech description
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Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
CB - Analytical chemistry, separation
OECD FORD branch
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Result continuities
Project
<a href="/en/project/GA14-06319S" target="_blank" >GA14-06319S: Development of microfluidic interfaces for multidimensional liquid phase separation/mass spectrometry analysis of complex biological samples</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2015
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Electrophoresis
ISSN
0173-0835
e-ISSN
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Volume of the periodical
36
Issue of the periodical within the volume
11-12
Country of publishing house
DE - GERMANY
Number of pages
5
Pages from-to
1344-1348
UT code for WoS article
000356004200014
EID of the result in the Scopus database
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