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Macroporous cryogel based spin column with immobilized concanavalin A for isolation of glycoproteins

The result's identifiers

  • Result code in IS VaVaI

    <a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216275%3A25310%2F15%3A39898280" target="_blank" >RIV/00216275:25310/15:39898280 - isvavai.cz</a>

  • Alternative codes found

    RIV/68081715:_____/15:00434034

  • Result on the web

    <a href="http://dx.doi.org/10.1002/elps.201400413" target="_blank" >http://dx.doi.org/10.1002/elps.201400413</a>

  • DOI - Digital Object Identifier

    <a href="http://dx.doi.org/10.1002/elps.201400413" target="_blank" >10.1002/elps.201400413</a>

Alternative languages

  • Result language

    angličtina

  • Original language name

    Macroporous cryogel based spin column with immobilized concanavalin A for isolation of glycoproteins

  • Original language description

    In this work we have developed a hydrophilic poly(hydroxyethyl methacrylate-co-poly(ethylene glycol) diacrylate) cryogel placed in the centrifugal filter device. The composition of the polymerization mixture as well as the polymerization conditions wereoptimized in order to prepare a material with bimodal pore size distribution with 20-50 ?m flow through macropores and submicrometer pores in the polymer walls. The optimized, mechanically stable, highly porous, material was used for spin column lectin chromatography. The surface of the monolithic scaffold was activated by epichlorohydrin and used for immobilization of concanavalin A to provide the affinity supports for selective isolation of glycoproteins containing high mannose glycan structures. Theperformance of the developed lectin modified cryogels was evaluated by analyses of glycoprotein mixtures. The efficiency and selectivity of the affinity supports were confirmed by MALDI-MS analysis.

  • Czech name

  • Czech description

Classification

  • Type

    J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

  • CEP classification

    CB - Analytical chemistry, separation

  • OECD FORD branch

Result continuities

  • Project

    <a href="/en/project/GA14-06319S" target="_blank" >GA14-06319S: Development of microfluidic interfaces for multidimensional liquid phase separation/mass spectrometry analysis of complex biological samples</a><br>

  • Continuities

    P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Others

  • Publication year

    2015

  • Confidentiality

    S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Data specific for result type

  • Name of the periodical

    Electrophoresis

  • ISSN

    0173-0835

  • e-ISSN

  • Volume of the periodical

    36

  • Issue of the periodical within the volume

    11-12

  • Country of publishing house

    DE - GERMANY

  • Number of pages

    5

  • Pages from-to

    1344-1348

  • UT code for WoS article

    000356004200014

  • EID of the result in the Scopus database