ON-CHIP ELISA ON MAGNETIC PARTICLES: ISOLATION AND DETECTION OF SPECIFIC ANTIBODIES FROM SERUM

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216275%3A25310%2F15%3A39900535" target="_blank" >RIV/00216275:25310/15:39900535 - isvavai.cz</a>
Result on the web
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DOI - Digital Object Identifier
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Result language
angličtina
Original language name
ON-CHIP ELISA ON MAGNETIC PARTICLES: ISOLATION AND DETECTION OF SPECIFIC ANTIBODIES FROM SERUM
Original language description
In recent years, microfluidics has shown considerable promise for improving diagnostics and biology research. Certain properties of microfluidic technologies, such as rapid sample processing and the precise control of fluids in an assay, have made them attractive candidates to replace traditional experimental approaches. Here, an example of such application is presented: ELISA that uses magnetic particles as the solid phase (MELISA) implemented into a microfluidic device. The microfluidic Rhombic chamber chip (120 ?l chamber volume, hydrophilized) in ChipGenie(R) edition-P holder (microfluidic ChipShop, Jena, D) with strong integrated magnets enabling to fix the magnetic particles in reaction chamber and arrange them as a fluidized bed was employed. The behaviour and colloidal stability of 11 various types of superparamagnetic particles varying in composition and diameter (0.5-100 ?m) were compared. Parameters as optimal amount of magnetic particles inside the chamber, rate of aggregat
Czech name
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Czech description
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Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>S - Specificky vyzkum na vysokych skolach

Publication year
2015
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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