Retention behavior of lipids in reversed-phase ultrahigh-performanceliquid chromatography-electrospray ionization mass spectrometry

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216275%3A25310%2F16%3A39901790" target="_blank" >RIV/00216275:25310/16:39901790 - isvavai.cz</a>

Result on the web

<a href="http://dx.doi.org/10.1016/j.chroma.2016.04.082" target="_blank" >http://dx.doi.org/10.1016/j.chroma.2016.04.082</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.chroma.2016.04.082" target="_blank" >10.1016/j.chroma.2016.04.082</a>

Result language

angličtina

Original language name

Retention behavior of lipids in reversed-phase ultrahigh-performanceliquid chromatography-electrospray ionization mass spectrometry

Original language description

Reversed-phase ultrahigh-performance liquid chromatography (RP-UHPLC) method using two 15 cmsub-2 m particles octadecylsilica gel columns is developed with the goal to separate and unambigu-ously identify a large number of lipid species in biological samples. The identification is performed by thecoupling with high-resolution tandem mass spectrometry (MS/MS) using quadrupole - time-of-flight(QTOF) instrument. Electrospray ionization (ESI) full scan and tandem mass spectra are measured inboth polarity modes with the mass accuracy better than 5 ppm, which provides a high confidence oflipid identification. Over 400 lipid species covering 14 polar and nonpolar lipid classes from 5 lipid cate-gories are identified in total lipid extracts of human plasma, human urine and porcine brain. The generaldependences of relative retention times on relative carbon number or relative double bond number areconstructed and fit with the second degree polynomial regression. The regular retention patterns inhomologous lipid series provide additional identification point for UHPLC/MS lipidomic analysis, whichincreases the confidence of lipid identification. The reprocessing of previously published data by our andother groups measured in the RP mode and ultrahigh-performance supercritical fluid chromatographyon the silica column shows more generic applicability of the polynomial regression for the description ofretention behavior and the prediction of retention times. The novelty of this work is the characterizationof general trends in the retention behavior of lipids within logical series with constant fatty acyl lengthor double bond number, which may be used as an additional criterion to increase the confidence of lipididentification.

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

CB - Analytical chemistry, separation

OECD FORD branch

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Project

<a href="/en/project/LL1302" target="_blank" >LL1302: Mass Spectrometry in Cancer Research: Lipid Biomarkers for Early Diagnostics</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>S - Specificky vyzkum na vysokych skolach<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2016

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Journal of Chromatography A

ISSN

0021-9673

e-ISSN

—

Volume of the periodical

1450

Issue of the periodical within the volume

June

Country of publishing house

NL - THE KINGDOM OF THE NETHERLANDS

Number of pages

10

Pages from-to

"76?85"

UT code for WoS article

000377323700009

EID of the result in the Scopus database

2-s2.0-84968750673