An HPLC method for the determination of selected amino acids in human embryo culture medium

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216275%3A25310%2F17%3A39910805" target="_blank" >RIV/00216275:25310/17:39910805 - isvavai.cz</a>

Result on the web

<a href="http://dx.doi.org/10.1002/bmc.3800" target="_blank" >http://dx.doi.org/10.1002/bmc.3800</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1002/bmc.3800" target="_blank" >10.1002/bmc.3800</a>

Result language

angličtina

Original language name

An HPLC method for the determination of selected amino acids in human embryo culture medium

Original language description

A method for the determination of selected amino acids in culture medium using HPLC with fluorescence detection is described. Twenty hours after intra-cytoplasmic sperm injection, one randomly selected zygote was transferred to the culture medium. After incubation (72 h after fertilization), the culture medium in which the embryo was incubated and blank medium was immediately stored at -80°C. Filtered medium samples were derivatized with ortho-phthalaldehyde (naphthalene-2,3-dicarboxaldehyde), forming highly fluorescent amino acids derivatives. Reverse-phase columns (LichroCART, Purospher STAR RP-18e or Ascentis Express C18) were used for the separation. The derivatives were analyzed by gradient elution with a mobile phase containing ethanol and sodium dihydrogen phosphate. The analytical performance of this method is satisfactory for all amino acids; the intra-assay coefficients of variation were &lt;10% and quantitative recoveries were between 95.5 and 104.4%. Changes in the levels of selected amino acids before and after human embryo cultivation were observed. After embryo incubation, the levels of all amino acids in the medium were increased, apart from aspartate and asparagine. After the cultivation of some embryos, amino acids which were not part of the medium were detected. Low amino acids turnover was observed in some embryos.

Czech name

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Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

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OECD FORD branch

10406 - Analytical chemistry

Project

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Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2017

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Biomedical Chromatography

ISSN

0269-3879

e-ISSN

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Volume of the periodical

31

Issue of the periodical within the volume

2

Country of publishing house

GB - UNITED KINGDOM

Number of pages

11

Pages from-to

"e3800-1"-"e3800-11"

UT code for WoS article

000397273800008

EID of the result in the Scopus database

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